Fig. S1

Co-injection of zebrafish embryos with morpholino antisense oligos (MOs) and the hybrid RNA encoding GFP fused to the 5′ untranslated and initial 20-bp coding region of their target genes. (A) Schematic of experimental procedures. The hybrid RNA or a mixture of the hybrid RNA and MOs was injected into one-cell stage embryos. The hybrid RNA activates GFP expression a couple of hours after injection (above), but simultaneous introduction of MOs represses this RNA-mediated GFP expression if MOs specifically inhibit the translation of their target genes (beneath). We examined GFP fluorescence in embryos injected with different combinations of RNAs and Mos: (B) no RNA and no MO; (C) the hybrid RNA encoding GFP fused to the 5′ untranslated and initial 20-bp coding region of the chk1 gene RNA(Chk1:GFP) and no MO; (D) RNA(Chk2:GFP) and no MO; (E) RNA(Chk1:GFP) and MO-Chk1; (F) RNA(Chk2:GFP) and MO-Chk2; (G) RNA(Chk1:GFP) and 5mis-MO-Chk1; (H) RNA(Chk2:GFP) and 5mis-MO-Chk2; (I) RNA(Chk1:GFP) and MO-Chk2; (J) RNA(Chk2:GFP) and MO-Chk1.