ZFIN ID: ZDB-FIG-080326-60
Pézeron et al., 2008 - Rasl11b knock down in zebrafish suppresses one-eyed-pinhead mutant phenotype. PLoS One   3(1):e1434 Full text @ PLoS One
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Anatomical Terms:
Stage: Shield
PHENOTYPE:
Fish:
Observed In:
Stage: Shield

Fig. 3 Opposite effects of Nodal and Oep on rasl11b expression. (A) Scheme of the Nodal cascade. The Oep coreceptor is necessary for the binding of the Nodal ligand Cyc and Sqt to the TGFβ receptors (containing the type I receptor Taram-A, the zebrafish Alk4 orthologue) that in turn phosphorylate Smad2. Smad2-P is translocated to the nucleus and triggers the transcription of a first set of genes encoding the transcription factors, Mixer, Gata5 and Mezzo. This set is required for the expression of the Sox factor Casanova that in turn initiates the transcription of the endodermal markers sox17 and axial/foxa2. (B–M, Dorsal view of gastrulae save C, I, sagittal sections and G, lateral view) (B, C) In wild-type (WT) embryos rasl11b is expressed at the animal pole (arrows) and at the dorsal margin (black arrowheads). (D) This marginal expression is lost in MZoep mutants (star), devoid of maternal and zygotic oep, consequently devoid of Nodal signaling, and so, unable to form most of the mesendoderm. (E, F) rasl11b dorso-pression is lost in MZoep mutants (star), devoid of maternal and zygotic oep, consequently devoid of Nodal marginal expression is normal in the cyclops Nodal mutant and largely reduced in the MZsquint Nodal mutant. (G) Activation of the Nodal signal by injection of a constitutively activated form of the Nodal type I receptor Taram-A (tar*) leads to a duplication of the rasl11b marginal expression domain (likely by inducing a second organizer, white arrowheads) (H, I) In Zoep mutants, devoid of zygotic oep, this mesendodermal expression domain is extended (white arrowheads). (J–M) A large series of embryos expressing different levels of Nodal signal was generated by injecting between blastula cells increasing doses of the recombinant Lefty protein, a Nodal pathway extracellular inhibitor. Here, only four representative doses are displayed. A progressive decrease of rasl11b marginal expressi

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
rasl11b WT standard conditions Shield margin ISH
Shield presumptive ectoderm ISH
Shield shield ISH
ndr1cz35/cz35 standard conditions Shield presumptive ectoderm ISH
Shield Not Detected shield ISH
ndr2m294/m294 standard conditions Shield margin ISH
Shield presumptive ectoderm ISH
Shield shield ISH
tdgf1tz257/tz257 standard conditions Shield margin ISH
tdgf1tz257/tz257 standard conditions Shield Not Detected margin ISH
Shield presumptive ectoderm ISH
tdgf1tz257/tz257 standard conditions Shield presumptive ectoderm ISH
tdgf1tz257/tz257 standard conditions Shield Not Detected shield ISH
tdgf1tz257/tz257 standard conditions Shield shield ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
ndr1cz35/cz35 standard conditions Shield shield morphology, abnormal
ndr2m294/m294 standard conditions Shield shield morphology, normal
tdgf1tz257/tz257 standard conditions Shield shield morphology, abnormal
tdgf1tz257/tz257 standard conditions Shield shield morphology, abnormal
Acknowledgments:
ZFIN wishes to thank the journal PLoS One for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS One