header logo image header logo text
Downloads Login
Research
General Information
ZIRC
ZFIN ID: ZDB-FIG-080326-63
Pézeron et al., 2008 - Rasl11b knock down in zebrafish suppresses one-eyed-pinhead mutant phenotype. PLoS One   3(1):e1434 Full text @ PLoS One
ADDITIONAL FIGURES
PHENOTYPE:
Fish:
Condition:
Knockdown Reagent:
Observed In:
Stage Range: 50%-epiboly to Bud

Fig. 6 rasl11b interacts specifically with oep but does not affect the Nodal/Smad2 transduction pathway. (A) The MZsqt, MZoep, Zoep, bon and cas mutants have a clear reduction of endodermal cells and so were used to quantify the putative impact of rasl11b knock down at different steps/levels of the nodal pathway. The Zoep mutant was the only one rescued by the rasl11b MO-ATG injection. This rescue was abolished by co-injection of rasl11b MO-resistant mRNA (rasl11bMOr). Error bars indicate standard deviation. (B, C) It is impossible to generate clutches of 100% Zoep embryos, and because one embryo cannot give enough material for both immunoblot and genotyping experiments, 100% Zoep-like mutant clutches were produced by injecting clutches of 100% MZoep eggs with low doses of wild-type oep mRNA. Half of them were then co-injected with rasl11b MO-ATG. Each batch was split in two, one used fant clutches were produced by injecting clutches of 100% MZoep eggs with low doses of wild-type oep mRNA. Half ofor phosphorylated Smad2 (Smad2-P) level analysis, the second for endodermal cell number count (assayed by sox17 in situ hybridization). Proteins were detected by western blotting using a Smad2-P antibody. Note that increasing doses of wild-type oep mRNA were correlated with an increase of Smad2-P and sox17 endodermal cell number, whereas co-injection with rasl11b MO-ATG increased endodermal cell number without generating more Smad2-P (Error bars indicate standard

Gene Expression Details No data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
mixl1m425/m425 standard conditions 50%-epiboly - Bud endoderm endodermal cell decreased amount, abnormal
mixl1m425/m425 + MO1-rasl11b standard conditions 50%-epiboly - Bud endoderm endodermal cell decreased amount, abnormal
ndr1cz35/cz35 standard conditions 50%-epiboly - Bud endoderm endodermal cell decreased amount, abnormal
ndr1cz35/cz35 + MO1-rasl11b standard conditions 50%-epiboly - Bud endoderm endodermal cell decreased amount, abnormal
sox32ta56/ta56 standard conditions 50%-epiboly - Bud endoderm aplastic, abnormal
sox32ta56/ta56 + MO1-rasl11b standard conditions 50%-epiboly - Bud endoderm aplastic, abnormal
tdgf1tz257/tz257 standard conditions 50%-epiboly - Bud endoderm aplastic, abnormal
tdgf1tz257/tz257 chemical treatment: messenger RNA 50%-epiboly - Bud endoderm endodermal cell decreased amount, abnormal
tdgf1tz257/tz257 + MO1-rasl11b chemical treatment: messenger RNA 50%-epiboly - Bud endoderm endodermal cell decreased amount, abnormal
tdgf1tz257/tz257 + MO1-rasl11b standard conditions 50%-epiboly - Bud endoderm aplastic, abnormal
Acknowledgments:
ZFIN wishes to thank the journal PLoS One for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS One