FIGURE

Fig. 5

ID

ZDB-FIG-080326-41

Publication

Yang et al., 2008 - cAMP/PKA Regulates Osteogenesis, Adipogenesis and Ratio of RANKL/OPG mRNA Expression in Mesenchymal Stem Cells by Suppressing Leptin

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Fig. 5

Leptin abrogates IBMX-induced lack of skeletal ossification in developing zebrafish. Embryos were treated in vehicle DMSO, IBMX (0.045 mM), and IBMX with human [H] or mouse [M] leptin (L1: 0.6 μg/mL, L2: 1.5 μg/mL) at 2 dpf. (A) Morphology of embryos at 8 dpf. (B) Alizarin Red S and alcian blue staining of whole zebrafish reveals normal skeletal ossification in DMSO-treated embryos at otolith (arrow), while absent skeletal ossification is evident in IBMX-treated embryos. The addition of human and mouse leptin both in low and high concentrations abrogates IBMX-induced absence of skeletal ossification. (C) Hematoxylin-and-eosin staining of zebrafish sections reveals impaired bone development in IBMX-treated embryos. Arrows indicate bone development with cell aggregation at otolith and pharyngeal arches. (D) Raman microspectroscopic analysis of bone development at otolith in zebrafish.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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