FIGURE

Fig. 9

ID

ZDB-FIG-071218-10

Publication

Tsujikawa et al., 2007 - Mechanism of positioning the cell nucleus in vertebrate photoreceptors

Other Figures

All Figure Page

Back to All Figure Page

Fig. 9

Photoreceptor Polarity and the Subcellular Distribution of the BBS4 Polypeptide. (A and B) Staining for a mitochondrial marker, Porin (green), and Actin-associated polypeptide Cortactin (red) at 3.25 dpf does not reveal obvious differences between wild-type (A) and mutant (B) cells. (C and D) Has/aPKC (green) and ZO-1 (red) polypeptides localize to narrow apical cell surface domains in the area of cell junctions, in wild-type cells (C) as well as in the clusters of elongated mutant photoreceptors (D). (E and F) Staining for BBS4 (green) and acetylated tubulin (red) in zebrafish photoreceptors at 3 dpf. In wild-type (E) but not mutant (F), BBS4 forms aggregates in the vicinity of connecting cilia (arrowheads). (G and H) Staining for BBS4 (green) and basal bodies (red) in zebrafish photoreceptors at 4 dpf. BBS4 staining is strongly reduced in lis1a morphants (H; compare with G). Cilia (E and F) and basal bodies (G and H) are visualized by using anti-acetylated tubulin and anti-g tubulin antibodies, respectively. In all panels, apical is up.

Expression Data

Gene:	bbs4
Fish:	AB dctn1a^m632/m632 (AB)
Anatomical Term:	eye photoreceptor cell
Stage Range:	Protruding-mouth to Day 4

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB + MO1-dctn1a AB + MO1-pafah1b1b + MO2-pafah1b1b
Knockdown Reagents:	MO1-dctn1a MO1-pafah1b1b MO2-pafah1b1b
Observed In:	apical protein localization
Stage Range:	Protruding-mouth to Day 4

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA