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ZIRC
ZFIN ID: ZDB-FIG-071218-10
Tsujikawa et al., 2007 - Mechanism of positioning the cell nucleus in vertebrate photoreceptors. Proc. Natl. Acad. Sci. USA   104(37):14819-14824 Full text @ Proc. Natl. Acad. Sci. USA
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Anatomical Term:
Stage Range: Protruding-mouth to Day 4
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage Range: Protruding-mouth to Day 4

Fig. 9 Photoreceptor Polarity and the Subcellular Distribution of the BBS4 Polypeptide. (A and B) Staining for a mitochondrial marker, Porin (green), and Actin-associated polypeptide Cortactin (red) at 3.25 dpf does not reveal obvious differences between wild-type (A) and mutant (B) cells. (C and D) Has/aPKC (green) and ZO-1 (red) polypeptides localize to narrow apical cell surface domains in the area of cell junctions, in wild-type cells (C) as well as in the clusters of elongated mutant photoreceptors (D). (E and F) Staining for BBS4 (green) and acetylated tubulin (red) in zebrafish photoreceptors at 3 dpf. In wild-type (E) but not mutant (F), BBS4 forms aggregates in the vicinity of connecting cilia (arrowheads). (G and H) Staining for BBS4 (green) and basal bodies (red) in zebrafish photoreceptors at 4 dpf. BBS4 staining is strongly reduced in lis1a morphants (H; compare with G). Cilia (E and F) and basal bodies (G and H) are visualized by using anti-acetylated tubulin and anti-g tubulin antibodies, respectively. In all panels, apical is up.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
bbs4 AB standard conditions Protruding-mouth eye photoreceptor cell IHC
Day 4 eye photoreceptor cell IHC
dctn1am632/m632(AB) standard conditions Protruding-mouth eye photoreceptor cell IHC
Day 4 eye photoreceptor cell IHC
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
AB + MO1-dctn1a standard conditions Protruding-mouth apical protein localization disrupted, abnormal
AB + MO1-pafah1b1b + MO2-pafah1b1b standard conditions Day 4 apical protein localization disrupted, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Proc. Natl. Acad. Sci. USA for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Proc. Natl. Acad. Sci. USA