PUBLICATION

Mechanism of positioning the cell nucleus in vertebrate photoreceptors

Authors
Tsujikawa, M., Omori, Y., Biyanwila, J., and Malicki, J.
ID
ZDB-PUB-070907-33
Date
2007
Source
Proceedings of the National Academy of Sciences of the United States of America   104(37): 14819-14824 (Journal)
Registered Authors
Malicki, Jarema, Omori, Yoshihiro, Tsujikawa, Motokazu
Keywords
Bardet?Biedl syndrome, retina, motor complex, neurodegeneration
MeSH Terms
  • Microtubule-Associated Proteins/metabolism
  • Nuclear Envelope/chemistry
  • Mutation
  • Mitogen-Activated Protein Kinases/chemistry
  • Mitogen-Activated Protein Kinases/genetics
  • Mosaicism
  • Animals
  • DNA Mutational Analysis
  • Protein Structure, Tertiary
  • RNA, Messenger/analysis
  • Zebrafish/embryology*
  • Zebrafish/physiology*
  • Cell Nucleus/physiology*
  • Zebrafish Proteins/metabolism
  • 1-Alkyl-2-acetylglycerophosphocholine Esterase/metabolism
  • In Situ Hybridization
  • Embryo, Nonmammalian
  • Genetic Linkage
  • Dyneins/metabolism
  • Models, Biological
  • Photoreceptor Cells, Vertebrate/cytology
  • Photoreceptor Cells, Vertebrate/physiology*
  • Green Fluorescent Proteins/metabolism
  • Immunohistochemistry
  • Cell Survival/physiology
  • Retina/cytology
  • Retina/embryology
(all 27)
PubMed
17785424 Full text @ Proc. Natl. Acad. Sci. USA
Abstract
Organelles are frequently distributed in a nonrandom manner in a cell's cytoplasm. A particular distribution pattern often facilitates a specific function of a cell, whereas its aberrations can lead to cell death. We show that a mutation in the zebrafish mikre oko (mok) locus, which encodes dynactin 1 subunit of the dynactin complex, produces a severe displacement of the photoreceptor cell nucleus toward the synaptic terminus. Interference with the function of other dynein complex constituents, including p50/dynamitin, the Lis1 polypeptide, and the disruption of a nuclear envelope component of the syne gene family in vertebrate photoreceptors also result in the mispositioning of nuclei. Although the overall photoreceptor polarity is not affected, this phenotype is accompanied by a misdistribution of the Bardet-Biedl syndrome 4 polypeptide and a decreased photoreceptor survival. These findings reveal an important mechanism that regulates nuclear position in vertebrate neurons.
Genes / Markers
Figures
Figure Gallery (5 images)
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Expression
Phenotype
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
m632
    		Point Mutation
    tz288
      		Point Mutation
      1 - 2 of 2
      Show
      Human Disease / Model
      No data available
      Sequence Targeting Reagents
      Target Reagent Reagent Type
      dctn1aMO1-dctn1aMRPHLNO
      pafah1b1bMO1-pafah1b1bMRPHLNO
      pafah1b1bMO2-pafah1b1bMRPHLNO
      syne2aMO1-syne2aMRPHLNO
      1 - 4 of 4
      Show
      Fish
      Fish
      AB
      AB + MO1-dctn1a
      AB + MO1-pafah1b1b + MO2-pafah1b1b
      AB + MO1-syne2a
      AB + MO2-pafah1b1b
      dctn1am632/m632 (AB)
      ift88tz288/tz288
      1 - 7 of 7
      Show
      Antibodies
      Orthology
      No data available
      Engineered Foreign Genes
      No data available
      Mapping
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      Citation
      Tsujikawa, M., Omori, Y., Biyanwila, J., and Malicki, J. (2007) Mechanism of positioning the cell nucleus in vertebrate photoreceptors. Proceedings of the National Academy of Sciences of the United States of America. 104(37):14819-14824.