Fig. 4

Expression of sema3Fa. A,C,E, and G are lateral views of whole-mount embryos and B,D,F, and H are stage-matched transverse sections. A,B: At 30 hpf, sema3Fa transcript is seen in the ventral retina (arrowhead), in the optic tectum, and in the ventral and dorsal regions of the posterior tuberculum. There is also label in the cerebellum. C,D: At 50 hpf, sema3Fa is expressed in the ventral posterior tuberculum and in bilateral nuclei in the dorsal posterior tuberculum. Expression in the ventral retina (arrowhead) continues. E,F: At 60 hpf, expression in the dorsal posterior tuberculum and cerebellum is maintained. G,H: At 72 hpf, there is diffuse label in the anterior brain (H). There is also expression in the jaw (E,G,H), pharyngeal arches (E,G). and the pectoral fin buds (E). cb, cerebellum; FB, fin bud; j, jaw; PA, pharyngeal aches; PTd, dorsal posterior tuberculum; PTv, ventral posterior tuberculum; tec, tectum. Scale bars in B,D,F, H = 50 μm.