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ZIRC
ZFIN ID: ZDB-FIG-071006-3
Arnaout et al., 2007 - Zebrafish model for human long QT syndrome. Proceedings of the National Academy of Sciences of the United States of America   104(27):11316-11321 Full text @ Proc. Natl. Acad. Sci. USA
ADDITIONAL FIGURES
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage: Long-pec

Fig. 4

Wild-type embryonic hearts in the Tg(cmlc2:gCaMP)s878 background exhibit atrial and ventricular conduction waves, whereas mutant hearts exhibit atrial, but no ventricular, conduction waves. SPIM videos of live 48 hpf hearts in the Tg(cmlc2:gCaMP)s878 background (see SI Movies 3 and 4) were processed to determine the fluorescence intensity of selected regions of the heart over time. Each selected region has a corresponding number plotted below. The dotted lines mark an arbitrary point in time to facilitate comparison across the different plots. (a) In a wild-type heart, fluorescence intensity varies with time in atrial and ventricular regions of the heart as the wave of depolarization propagates through the heart. This wave represents a wild-type heart rhythm. (b) In a kcnh2s290 mutant heart, fluorescence intensity varies in the atrium, but the ventricle maintains a constant, low-level intensity. The mutant heart lacks a ventricular conduction wave.

Gene Expression Details No data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
kcnh6as290/s290; s878Tg + MO1-tnnt2a standard conditions Long-pec cardiac ventricle conductivity, abnormal
Long-pec heart contraction arrested, abnormal
s878Tg + MO1-tnnt2a standard conditions Long-pec heart contraction arrested, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Proceedings of the National Academy of Sciences of the United States of America for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Proc. Natl. Acad. Sci. USA