FIGURE

Fig. S3

ID

ZDB-FIG-070918-64

Publication

Walker et al., 2006 - Zebrafish furin mutants reveal intricacies in regulating Endothelin1 signaling in craniofacial patterning

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Fig. S3

furinA and furinB MOs specifically and efficiently block splicing. RT-PCR was performed on RNA isolated from 24 and 48 hpf zebrafish embryos injected with 16 ng furinA MO or 10 ng of furinB MO at 1–4 cell stage. These MOs are designed to exon 9 splice sites. The following gene-specific PCR primers were used: furinA, 5′GTGGATGGGCCTGCAAAAT3′/5′CTTTGCTAAGGCTACAATG3′& and furinB, 5′TCTAAGCTTGAACTCCCAGC3′/5′CTGGGCCAAAGCGACCATTG3′. Arrowheads indicate wild-type splice products, and asterisks indicate an MO-induced splice variant with deletion of exon 9.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 295(1), Walker, M.B., Miller, C.T., Talbot, J.C., Stock, D.W., and Kimmel, C.B., Zebrafish furin mutants reveal intricacies in regulating Endothelin1 signaling in craniofacial patterning, 194-205, Copyright (2006) with permission from Elsevier. Full text @ Dev. Biol.