Fig. 6

Nkds can antagonize ectopic Wnt8. (A) Overexpression of Wnt8 in wild-type (wt) embryos results in an eyeless phenotype. (B) Co-expression of wnt8 and nkd1 or nkd2 results in a near complete suppression of the Wnt8 effect. This is quantified in panel C. (D–G) boz mutants have ectopic Wnt8 activity in the dorsal mesoderm, which results in a reduction of axial tissue as demonstrated by reduced ntl expression (D), or in the physical absence of a notochord (F). The presumptive neuroectoderm is labeled with six3b which is also reduced in boz mutants (D). Injection of nkd1 or nkd2a into boz embryos suppresses the deficiency of ntl expression in the notochord (E) and of a notochord at 24 hpf (G). Error bars represent standard deviation (Students t-test; ***, p < 0.0001). (A, B, F, G) Lateral view, anterior to the left; (D, E) dorsal view.