Normal Expression of per4 in aal Mutants. Whole-mount in situ hybridization with probes against per4 of aal mutants and wild-type siblings. Mutants and wild-type siblings were discriminated by co-hybridizing with a probe against growth hormone (unpublished data). (A–D) Overview of staining results for mutants (C and D) and wild-type siblings (A and B) at ZT 12 (A and C) and 23 (B and D). (E and F) Close-ups of mutant and wild-type sibling larvae at ZT12 (E) and ZT23 (F). Strong per4 staining is visible in both mutant and wild-type siblings at ZT23, whereas barely any staining can be detected in larvae of both genotypes at ZT12.
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