Fig. S2

Normal Expression of Hypothalamic Markers in rx3 Mutants. Whole-mount in situ hybridization with probes against somatostatin3 (ppss3) [68] (A), isotocin (itnp) [67] (B), and corticotropin releasing factor (crf) (C) of strong and weak rx3 mutants and wild-type siblings on 6 dpf. For each panel: wild-type (“wildtype”), strong mutant (“strong”), and weak mutant (“weak”); top row shows a lateral view with the anterior end left (in wild-type: left eye removed to allow a better view of the brain stainings), and the bottom row shows a dorsal view with the anterior end left. Expression patterns of these representative probes appear normal, despite the lack of normal eyecups in the mutants.