FIGURE

Fig. 7

ID

ZDB-FIG-070628-6

Publication

Lin et al., 2007 - Depletion of Med10 enhances Wnt and suppresses Nodal signaling during zebrafish embryogenesis

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Fig. 7

The multiple tail bud phenotype of med10 morphants is recapitulated in med13/med15 double morphants. Injection of MO1-Med13 (10 ng) expands Wnt target-gene expression. Shown are spr2 and cdx4 staining in injected (F,J) and control (E,I) embryos. The expression of cas (A,B) serves as a control. Injection of MO1-Med15 (10 ng) suppresses Nodal target gene cas expression (D), but does not affect the expression of spr2 (H), as compared to control embryos (C,G). Mesoendoderm cells, as detected by staining for cas, are expressed in a region more close to the animal pole in med15 morphants (5 ng of MO1-Med15 injection) (L), as compared to control embryos (K). Shown in (A–D) are animal pole views of 30% epiboly embryos. Shown in (E–L) are lateral views of 75% epiboly embryos. (M–P) Reduction of both Med13 and Med15 (15 ng of MO1-Med13 and 3 ng of MO1-Med15) recapitulates the multiple tail bud phenotype of med10 morphants, as shown by tbx6 (N) and eve1 (P) staining. Shown are dorsal views. Arrowheads indicate the position of the ectopic tail buds.

Expression Data

Genes:	cdx4 sox32 sp5l
Fish:	WT WT + MO1-med13a WT + MO1-med15
Knockdown Reagents:	MO1-med13a MO1-med15
Anatomical Terms:	endoderm margin mesoderm
Stage Range:	30%-epiboly to 75%-epiboly

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB + MO1-med15 AB + MO1-med13a + MO1-med15
Knockdown Reagents:	MO1-med13a MO1-med15
Observed In:	dorsal/ventral pattern formation mesendoderm development tail bud
Stage Range:	30%-epiboly to Bud

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 303(2), Lin, X., Rinaldo, L., Fazly, A.F., and Xu, X., Depletion of Med10 enhances Wnt and suppresses Nodal signaling during zebrafish embryogenesis, 536-548, Copyright (2007) with permission from Elsevier. Full text @ Dev. Biol.