Distinct Expression Pattern of scl-α and -β during Early Embryonic Development (A–L) Examination of scl-α and -β expression by WISH in wild-type and mon mutant embryos. WISH was performed in 2-somite (s) (A and G), 4-somite (B and H), 8-somite (C and I), 18-somite (D and J), and 26-hpf (E and K) wild-type embryos, and 18-somite mon mutant embryos (F and L). The 3′-probe (A–F) detected the expression of both scl-α and -β, whereas the 5′-probe (G–L) detected scl-α expression. The arrows indicate the expression of scl-β (E), but not scl-α (K), in the ventral wall of DA. Embryos are in dorsal (A and G), flat-mounted dorsal (B, C, H, and I), and lateral (D–F and J–L) views with anterior to the top (A and G) or the left (B–F and H–L). (M–R) Transverse sections through the trunk region of 26-hpf wild-type embryos with dorsal up. Fluorescence in situ hybridization showed the expression of c-myb in the ventral wall of DA (white arrowhead in [M, O, P, and R]). Immunochemistry staining showed that Ab-Scl-C (black arrowhead in [N and O]) but not Ab-Scl-N (Q) detected Scl expression in the ventral wall of DA, demonstrating the co-localization of scl-β and c-myb in the ventral wall of DA (O). NC, notochord; NT, neural tube; PCV, posterior cardinal vein. (S) Schematic diagram in flat-mounted dorsal view to illustrate the temporal and spatial expression of scl-α and -β. Red represents scl-β expression, whereas yellow indicates scl-α/β co-expression.
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