Fig. 5

Mib^m132 and Mib^ta52b behave dominant-negatively. (A) Somite phenotype at 22ss of (a) control wt embryos, (b) mib(m132) mRNA-injected, and (c) mib(ta52b) mRNA-injected wt embryos; (d) mib(m132) mRNA-injected and (e) mib(ta52b) mRNA-injected embryos from incrosses of mib^tfi91 carriers. (B) 3dpf tail and pigmentation phenotypes of (a) control wt embryos; (b) control mib^tfi91 embryos; (c) type I and (c2) type II mib(m132)-injected wt embryos; (d) type I and (d′) type II mib(ta52b)-injected wt embryos; (e) type I, (e′) type II, and (e″) type III mib(m132)-injected embryos from incrosses of mib^tfi91 carriers; (f) type I, (f′) type II, and (f″) type III mib(ta52b)-injected embryos from incrosses of mib^tfi91 carriers. Note that type I: normal somite, curly-up tail and normal tail pigment; type II: abnormal somite, curly-up tail and normal tail pigment; and type III: abnormal somite, curly-up white tail. The numbers in the right upper corner indicate the frequency of each type (Table 3). (C) huC expression at 10ss in dorsal view of (a) control wt embryos, (b) control mib^tfi91 embryos, and (c) mib(m132)-injected wt embryos; (d) mib(m132)-injected and (e) mib(ta52b)-injected embryos from incrosses of mib^tfi91 carriers. (D) her4 expression at 22hpf of (a–a″) control wt embryos and (b–b″) control mib^tfi91 embryos; (c–c″) mib(m132)-injected and (d–d3) mib(ta52b)-injected embryos from incrosses of mib^tfi91 carriers; (a–d) head region, dorsal view; (a′–d′) trunk region and (a″–d″) tail region around cloaca, both lateral view.