Fig. S1
Antisense morpholino oligonucleotides block Foxd3 protein expression at all stages examined. Confocal images of immunocytochemistry of control (A, C, E) and morpholino-injected (B, D, F) embryos. (A, B) Dorsal view of 80% epiboly stage embryos. Arrows in panel (A) show Foxd3 expression in the dorsal margin and involuting axial mesoderm. (C, D) Ventral view of embryos at 36hpf. The yolk has been removed to image the anterior gut tube. Arrows in panel (C) show migrating neural crest-derived enteric precursors. (E, F) Lateral view of tail segments of embryos at 72 hpf. Arrows in panel (E) show lateral line nerve glial cells along the horizontal myoseptum. |
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Stage Range: | 75%-epiboly to Protruding-mouth |
Reprinted from Developmental Biology, 290(1), Lister, J.A., Cooper, C., Nguyen, K., Modrell, M., Grant, K., and Raible, D.W., Zebrafish Foxd3 is required for development of a subset of neural crest derivatives, 92-104, Copyright (2006) with permission from Elsevier. Full text @ Dev. Biol.