Epidermal expression of rbp4 is down-regulated following treatment with PTU (1-phenyl-2-thio-urea). A-F: Embryos and larvae were raised, from fertilization to 10 days postfertilization (dpf), in water without (A,C,E) or with (B,D,F) added PTU to prevent pigment formation. A-F: Whole-mount in situ hybridizations are shown in lateral views and anterior to the left with digoxigenin-labeled sense (A,B) and antisense (C-F) rbp4 riboprobes. E,F: Parasagittal sections after whole-mount in situ hybridization. C-F: Epidermal rbp4 transcript hybridization signal was strongly reduced in the epidermis of PTU-treated larva (D,F) in comparison with untreated animals (C,E). E,F: The outline of the liver is traced in red. Inserts in E and F are enlarged views of the anal papilla region. e, epidermis; l, liver; ap, anal papilla. Scale bars = 250 μm.
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