FIGURE

Fig. 4

ID

ZDB-FIG-061121-40

Publication

Flores et al., 2006 - A hierarchy of Runx transcription factors modulate the onset of chondrogenesis in craniofacial endochondral bones in zebrafish

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Fig. 4

Loss-of-function analyses using antisense morpholinos demonstrate which Runx proteins are essential at the onset of endochondral bone formation in the head skeleton. Embryos were injected with optimal amounts of MO at the 1-4 cell stage to inhibit gene function, and cartilage development was visualized with alcian blue staining at 3.5 (A-F) and 5 (G-L) dpf. (A,G) runx2bMM; (B,H) runx1MO; (C,I) runx2aMO; (D,J) runx2bT1MO; (E,K) runx2bT2MO; (F,L) runx3MO. Compared to runx2bMM controls, embryos treated with runx2bT1MO, runx2bT2MO, and runx3MO display significant reductions in chondrocyte differentiation at 3.5dpf (D-F). By 5dpf, runx2T1 morphants recover from an early delay in chondrogenesis (J), but those injected with runx2bt2MO and runx3MO show persistence of cartilaginous abnormalities with underdeveloped and reduced cartilage (K,L). The head skeleton of runx1 and runx2a morphants are mildly misshapen at 3.5dpf (B,C), and these become morphologically normal by 5dpf (H,I). Scale bars = 100 μm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-runx2a WT + MO1-runx3 WT + MO4-runx1 WT + MO5-runx2b
Knockdown Reagents:	MO1-runx2a MO1-runx3 MO4-runx1 MO5-runx2b
Observed In:	chondrocyte differentiation cranium
Stage Range:	Protruding-mouth to Day 5

Phenotype Detail

Acknowledgments

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