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ZFIN ID: ZDB-FIG-060410-11
Bellipanni et al., 2006 - Essential and opposing roles of zebrafish β-catenins in the formation of dorsal axial structures and neurectoderm. Development (Cambridge, England)   133(7):1299-1309 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: 1-cell to Prim-5

Fig. 3 Maternal β-catenin-2 transcript is reduced in ichabod embryos. (A) RT-PCR assay of RNA expression levels in wild-type and ichabod embryos. For each stage shown, oligo dT-primed cDNA was used as a template for three separate PCR amplifications using primers for β-catenin-1, β-catenin-2 and ef1α. Maternal β-catenin-2 transcript was reduced in ichabod embryos, but reduction of maternal β-catenin-1 transcript or zygotic transcripts of either gene was not observed. (B-M) Whole-mount in situ hybridization showing ubiquitous expression of both β-catenin genes and low expression of maternal β-catenin-2. One-cell stage (top row: B,E,H,K), sphere stage (middle row: C,F,I,L) and 90% epiboly (bottom row: D,G,J,M) wild-type (B-D, H-J) and ichabod (E-G, K-M) embryos were tested for expression of β-catenin-1 (B-G) and β-catenin-2 (H-M). Embryos in D,J are shown in lateral view with dorsal towards the right.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
ctnnb1 brsb2(AB) standard conditions 1-cell whole organism ISH
1-cell to Prim-5 whole organism RTPCR
Sphere whole organism ISH
90%-epiboly axis ISH
90%-epiboly whole organism ISH
ctnnb2p1 standard conditions 1-cell whole organism ISH
1-cell to Prim-5 whole organism RTPCR
Sphere whole organism ISH
90%-epiboly whole organism ISH
ctnnb2 brsb2(AB) standard conditions 1-cell whole organism ISH
1-cell to Prim-5 whole organism RTPCR
Sphere whole organism ISH
90%-epiboly axis ISH
90%-epiboly whole organism ISH
ctnnb2p1 standard conditions 1-cell whole organism ISH
1-cell to Prim-5 whole organism RTPCR
Sphere whole organism ISH
90%-epiboly whole organism ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development