ZFIN ID: ZDB-FIG-060410-11 |
Fig. 3 Maternal β-catenin-2 transcript is reduced in ichabod embryos. (A) RT-PCR assay of RNA expression levels in wild-type and ichabod embryos. For each stage shown, oligo dT-primed cDNA was used as a template for three separate PCR amplifications using primers for β-catenin-1, β-catenin-2 and ef1α. Maternal β-catenin-2 transcript was reduced in ichabod embryos, but reduction of maternal β-catenin-1 transcript or zygotic transcripts of either gene was not observed. (B-M) Whole-mount in situ hybridization showing ubiquitous expression of both β-catenin genes and low expression of maternal β-catenin-2. One-cell stage (top row: B,E,H,K), sphere stage (middle row: C,F,I,L) and 90% epiboly (bottom row: D,G,J,M) wild-type (B-D, H-J) and ichabod (E-G, K-M) embryos were tested for expression of β-catenin-1 (B-G) and β-catenin-2 (H-M). Embryos in D,J are shown in lateral view with dorsal towards the right. |
Gene | Antibody | Fish | Conditions | Stage | Anatomy | Assay |
---|---|---|---|---|---|---|
ctnnb1 | brsb2(AB) | standard conditions | 1-cell | whole organism | ISH | |
1-cell to Prim-5 | whole organism | RTPCR | ||||
Sphere | whole organism | ISH | ||||
90%-epiboly | axis | ISH | ||||
90%-epiboly | whole organism | ISH | ||||
ctnnb2p1 | standard conditions | 1-cell | whole organism | ISH | ||
1-cell to Prim-5 | whole organism | RTPCR | ||||
Sphere | whole organism | ISH | ||||
90%-epiboly | whole organism | ISH | ||||
ctnnb2 | brsb2(AB) | standard conditions | 1-cell | whole organism | ISH | |
1-cell to Prim-5 | whole organism | RTPCR | ||||
Sphere | whole organism | ISH | ||||
90%-epiboly | axis | ISH | ||||
90%-epiboly | whole organism | ISH | ||||
ctnnb2p1 | standard conditions | 1-cell | whole organism | ISH | ||
1-cell to Prim-5 | whole organism | RTPCR | ||||
Sphere | whole organism | ISH | ||||
90%-epiboly | whole organism | ISH |
Acknowledgments: | ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development |
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