Neuroectodermal phenotype of oep mutant embryos. (A,B) Expression of pax-a (arrowhead) in the forebrain, pax-b (arrow) at the midbrainhindbrain boundary) and krox-20 (stars) in rhombomeres 3 and 5 in wild-type (A) and oep mutant (B) embryos at the 10-somites stage. Note the apparently normal anterior-posterior organization of expression patterns. (C,D) Expression of shh in wild-type (C) and oep mutant (D) embryos at the 1-somite stage. Note the absence of shh expression in the head region (arrowhead) of oep mutants. shh is expressed in the notochord, but expression in the floor plate is reduced or absent (arrow) in oep mutant embryos. (E,F) Expression of shh in the head region of wild-type (E) and oep mutant (F) embryos at 26 hpf. shh is only found in the dorsal-most region next to the epiphysis in oep mutants (arrow). (G,H) Expression of nk2.2 in the head region of wild-type (G) and oep mutant (H) embryos at the 18-somites stage. Arrowhead highlights expression in the ventral diencephalon. shh and nk2.2 are expressed in adjacent domains, shh being the more ventral marker. (I-L) Expression of shh in the tail and trunk region of wild-type (I) and oep mutant (J-L) embryos at 26-28 hpf. Note that the floor plate (arrow) is lost (J) or reduced (K,L) in oep mutants. Some oep mutant embryos (L) show a more persistent expression of shh in the notochord (arrowhead).
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