ihha and col10a1 expression in rostro-caudal transverse sections of the head regions of 4dpf zebrafish larvae. A-F: In situ hybridization with an anti sense RNA probe corresponding to ihha (A) and col10a1 (B-F). A: Earliest expression of ihha detected in the skeleton is located in chondrocytes of the parachordal cartilage (pc), which also express col10a1 (B). The latter is more widely expressed, and transcripts were detected in forming dermal bones (C-F). Parasphenoid (ps), ectopterygoid (ecp), opercle (op), cleithrum (cl), and two bilateral clusters of cells, which are part of the pectoral girdle (arrows on F). ect, ectopterygoid; hb, hindbrain; n, notochord; opv, optic vesicle; ov, otic vesicle; pc, parachordal cartilage; ph, pharynx; ps, parasphenoid.
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