ZFIN ID: ZDB-FIG-060111-1
Bisgrove et al., 2005 - Polaris and Polycystin-2 in dorsal forerunner cells and Kupffer's vesicle are required for specification of the zebrafish left-right axis. Developmental Biology   287(2):274-288 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: 256-cell to Protruding-mouth

Fig. 1 Polaris and pkd2 are expressed in multiple ciliated tissues during early development. In situ localization of polaris (A–I) and pkd2 (J–R) transcripts at early cleavage through 72 hpf (all panels are lateral views except panels C, G, I, and P, dorsal views, and panel R, ventral view). Maternal polaris RNA is present in cleavage stage embryos (A) and becomes undetectable by early gastrula stages (B). At late gastrula stages (C), polaris expression is restricted to dorsal forerunner cells (dfc) which later aggregate to form the primordium of Kupffer′s vesicle (Kv) at the end of gastrulation (D). Polaris expression is ubiquitous at later stages with high levels detected in the otic vesicle (ov) (F), the pronephric duct primordia (pnd) and floorplate (fp) (G). At 24 hpf, polaris is expressed in the brain and in the caudal floorplate (H). At 3 dpf, polaris is expressed in the brain (I). Maternal pkd2 message is not detected by in situ hybridization (J). At 40% epiboly, zygotic expression is detected in a band of 3–4 cell diameters at the blastoderm margin (K). Pkd2 RNA is localized to the hypoblast of the dorsal midline and to DFCs during gastrulation (L). From tailbud stage through 24 hpf, pkd2 is ubiquitous with higher levels of expression in KV (M) that persist to the 6 somite stage (N). pkd2 message is also detected in the pronephric duct primordia and floorplate (P) and at 24 hpf is highly expressed in the brain (Q). At 3 dpf, pkd2 is expressed in the pharyngeal arches (pa) and pectoral fins (pf) (R).

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
ift88 AB standard conditions 512-cell whole organism ISH
30%-epiboly Not Detected whole organism ISH
75%-epiboly forerunner cell group ISH
1-4 somites forerunner cell group ISH
5-9 somites Not Detected Kupffer's vesicle ISH
10-13 somites otic placode ISH
10-13 somites pronephric duct ISH
Prim-5 brain ISH
Prim-5 floor plate ISH
Protruding-mouth brain ISH
Protruding-mouth eye ISH
pkd2 AB standard conditions 256-cell whole organism ISH
30%-epiboly margin ISH
75%-epiboly forerunner cell group ISH
75%-epiboly hypoblast ISH
Bud to Prim-5 Kupffer's vesicle ISH
Bud to Prim-5 whole organism ISH
10-13 somites floor plate ISH
10-13 somites pronephric duct ISH
Prim-5 brain ISH
Protruding-mouth pectoral fin ISH
Protruding-mouth pharyngeal arch ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 287(2), Bisgrove, B.W., Snarr, B.S., Emrazian, A., and Yost, H.J., Polaris and Polycystin-2 in dorsal forerunner cells and Kupffer's vesicle are required for specification of the zebrafish left-right axis, 274-288, Copyright (2005) with permission from Elsevier. Full text @ Dev. Biol.