FIGURE

Fig. 3

ID

ZDB-FIG-050929-1

Publication

Wang et al., 2005 - Hepatic and extrahepatic expression of vitellogenin genes in the zebrafish, Danio rerio

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Fig. 3

Northern blot analysis of tissue distribution of vtg mRNAs in both female and male zebrafish with or without E₂ treatment. Total RNAs were prepared from selected tissues as indicated at the top of each lane: B, brain; I, intestine; E, eye; O, ovary; T, testis; S, skin; G, gill; L, liver; M, muscle; F, whole fish. 10 μg of total RNA was loaded each lane. Blots were first hybridized with ³²P-labeled vtg probes and stripped and reprobed with a β-actin cDNA probe to ensure even loading and integrity of RNAs. Representative blots with the β-actin probe are shown at the bottom and consistently the level of β-actin mRNA is lower in the liver than in other tissues (Wang et al., 2000 and Mudumana et al., 2004). No vtg hybridization signal was detected in any tissue in control male zebrafish (not shown). The positions of 28S and 18S ribosomal RNAs are marked on the left and names of vtg transcripts with estimated molecular weights on the right. Weak signals in ovary total RNA are marked by arrowheads.

Expression Data

Genes:	vtg1 vtg2 vtg3 vtg4 vtg5 vtg6 vtg7
Fish:	WT
Condition:	chemical treatment: 17beta-estradiol
Anatomical Terms:	liver ovary skeletal muscle cell testis
Stage:	Adult

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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Reprinted from Gene, 356, Wang, H., Tan, J.T., Emelyanov, A., Korzh, V., and Gong, Z., Hepatic and extrahepatic expression of vitellogenin genes in the zebrafish, Danio rerio, 91-100, Copyright (2005) with permission from Elsevier. Full text @ Gene