FIGURE

Fig. 2

ID

ZDB-FIG-050922-9

Publication

Barresi et al., 2005 - Hedgehog regulated Slit expression determines commissure and glial cell position in the zebrafish forebrain

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Fig. 2

Gfap+ cells span the midline prior to formation of forebrain commissures. (A-F) Double labeling for axons (anti-AT; green) and Gfap+ cells (red). (A) Gfap+ cells span the midline posterior to the optic recess (dashed line) at the presumptive POC at 15 hpf. (B) By 18 hpf, Gfap+ cells span the midline, anterior and posterior to the optic recess (dashed line), at the presumptive AC and POC. (C) By 24 hpf, more distinct glial bridges (brackets) have formed at the presumptive AC and the forming POC (arrow). (D) At 28 hpf both commissures (arrows) have formed in association with the glial bridges. (E) The first RGC axons (arrowheads) grow towards the midline along Gfap+ cells at 34 hpf. (F) The optic chiasm (arrowhead) forms in association with a distinct cluster of Gfap+ fibers just anterior to the POC (bracket). At 15 hpf and 18 hpf, no axons are present in the forebrain (these data) (Wilson et al., 1990), so the green channel was omitted to reduce background. All panels show frontal views of the forebrain. Scale bar: 10 μm.

Expression Data

Gene:	gfap
Fish:	WT
Anatomical Term:	forebrain
Stage Range:	10-13 somites to Prim-15

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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