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ZFIN ID: ZDB-FIG-050902-1
Holzschuh et al., 2005 - Requirements for endoderm and BMP signaling in sensory neurogenesis in zebrafish. Development (Cambridge, England)   132(16):3731-3742 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage: Long-pec
PHENOTYPE:
Fish:
Observed In:
Stage: Long-pec

Fig. 3 Epibranchial defects in mutants that disrupt endoderm. Lateral views, anterior to the left. (A,B) Wild type and cas mutant at 48 hpf, immunolabeled with anti-zn8 antibody, which labels pharyngeal pouches 1-6. (C,D) Wild type and cas mutant at 48 hpf, immunolabeled with anti-Hu to mark sensory neurons. (E,F) Wild type and cas mutant at 48 hpf labeled by whole-mount in situ hybridization for phox2b mRNA in neural progenitors. (G,H) Examples of phox2b expression in vgo mutants. Arrow in G denotes a ventrally extended ganglion; arrows in H denote fused or missing ganglia. (I,J) her5:egfp expression in living wild type and vgo mutant at 24 hpf. Arrows in I denote contacts between pouches and ectoderm; arrows in J denote deformed or missing pouches. 1-5, pharyngeal pouches; gM, medial portion of vagal; gP, posterior lateral line; gV, trigeminal; gIX, glossopharyngeal; gX, vagal; hb, hindbrain; mb, midbrain.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
alcama WT standard conditions Long-pec pharyngeal pouch IHC
sox32ta56/ta56 standard conditions Long-pec Not Detected pharyngeal pouch IHC
phox2bb WT standard conditions Long-pec facial ganglion ISH
Long-pec statoacoustic (VIII) ganglion ISH
Long-pec vagal ganglion 1 ISH
Long-pec vagal ganglion 2 ISH
Long-pec vagal ganglion 3 ISH
Long-pec vagal ganglion 4 ISH
sox32ta56/ta56 standard conditions Long-pec facial ganglion ISH
Long-pec Not Detected glossopharyngeal ganglion ISH
Long-pec Not Detected vagal ganglion 1 ISH
Long-pec Not Detected vagal ganglion 2 ISH
Long-pec Not Detected vagal ganglion 3 ISH
Long-pec vagal ganglion 4 ISH
tbx1tm208/tm208 standard conditions Long-pec facial ganglion ISH
Long-pec statoacoustic (VIII) ganglion ISH
Long-pec vagal ganglion 1 ISH
Long-pec vagal ganglion 2 ISH
Long-pec vagal ganglion 4 ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
tbx1tm208/tm208 standard conditions Long-pec endoderm development disrupted, abnormal
Long-pec epibranchial ganglion fused with epibranchial ganglion, abnormal
Long-pec epibranchial ganglion increased size, abnormal
Long-pec glossopharyngeal ganglion absent, abnormal
Long-pec pharyngeal pouch aplastic, abnormal
Long-pec pharyngeal pouch malformed, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development