Expression of markers for neural crest and collagen in wild-type and mutant embryos. (A-D) Dorsal view of snail1b expression at three-somite stage showing decreased expression in sox9bb971 and double mutant; (E-T) lateral views; (E-H) dlx2a expression at 24 hpf slightly reduced in sox9bb971 and double mutant; (I-L) col2a1 expression at 68 hpf showing lack of scapulocoracoid in sox9a mutant and double mutant and smaller endochondral disc in sox9bb971 and double mutant; (M-T) sox10 expression in trunk and tail of 26 hpf embryos showing decreased expression. (A,E,I,M) Wild type; (B,F,J,N,R) sox9a- mutant; (C,G,K,O,S) sox9bb971; (D,H,L,P,T) double mutant. (Q-T) Higher magnification of M-P. Abbreviations: co, scapulocoracoid; ed, endochondral disc; ov, otic vesicle. Scale bars: in D 100 µm for A-D; in H, 100 µm for E-H; in L, 100 µm for I-L; in P, 100 µm for M-P; in T, 100 µm for Q-T.
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