ZFIN ID: ZDB-FIG-050307-5
Amoyel et al., 2005 - Wnt1 regulates neurogenesis and mediates lateral inhibition of boundary cell specification in the zebrafish hindbrain. Development (Cambridge, England)   132(4):775-785 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage: Long-pec
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage: Long-pec

Fig. 5 Neurogenesis at 48 hours in wnt MO-injected embryos. (A,B) Reticulospinal neurons labelled by RMO-44 antibody (arrows) are still present (11/14 embryos), and cranial motor nerves (V, VI, VII, IX and X) labelled with tbx20 (C,D) are mildly hypomorphic (11/11 embryos). The number of dbx1a-expressing cells appears to be similar in uninjected and morphant embryos (E,F), but their distribution in stripes adjacent to boundaries is disrupted (20/20 embryos). Sections of uninjected (I,J) and wnt1MO embryos (K,L) show that in uninjected embryos there are many neurons expressing dbx1a in the mantle zone (MZ); in the morphants, most dbx1a expression occurs in the ventricular zone (VZ) of progenitors (4/4 embryos), as determined by DAPI stain (J,L) and by absence of Hu staining (not shown). (G,H) gfap expression occurs in stripes adjacent to boundaries in uninjected embryos (arrows in G), and ectopically in wnt1 MO embryos (H, 12/13 embryos), suggesting that neural progenitors have switched fate to glial cells. Scale bar: in A, 50 µm for A,B,I-L; in C, 100 µm for C-H.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
dbx1a WT standard conditions Long-pec hindbrain ISH
WT + MO1-wnt1 standard conditions Long-pec hindbrain ISH
gfap WT standard conditions Long-pec hindbrain ISH
WT + MO1-wnt1 standard conditions Long-pec hindbrain ISH
tbx20 WT standard conditions Long-pec cranial ganglion ISH
WT + MO1-wnt1 standard conditions Long-pec cranial ganglion ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-wnt1 standard conditions Long-pec cranial nerve IX hypoplastic, abnormal
Long-pec cranial nerve VI hypoplastic, abnormal
Long-pec cranial nerve VII hypoplastic, abnormal
Long-pec cranial nerve X hypoplastic, abnormal
Long-pec glial cell mislocalised, abnormal
Long-pec motor neuron decreased amount, abnormal
Long-pec neurogenesis disrupted, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development