Enhancer Trap Construct

Et(hsp70l:GFP-GAL4FF)

ID

ZDB-ETCONSTRCT-080520-2

Previous Names

T2KhspGGFF (1)
Et(hsp70l:GFP-Gal4FF)
Et(hsp70l:GFP-Gal4FF-OlTol2)

Type

engineered_plasmid

Regulatory Regions

hsp70l (1)

Coding Sequences

GAL4FF (1)
GFP (1)

Contains Sequences

TOL2

Note

Enhancer trap construct incorporating medaka Tol2 transposable element. A heat-shock promoter drives expression of the DNA-binding domain from Gal4 and two transcription activating modules from VP16 fused to GFP.

Plasmid Map

Genomic Features

That Utilize Et(hsp70l:GFP-GAL4FF) Construct

Genomic Feature	Affected Genomic Regions
nk10bEt	gria4b
nk10cEt
nk14aEt
nk14bEt
nk14cEt
nk15aEt	skib
nk17aEt	zgc:92242
nk17bEt	ctbp2a
nk17cEt
nk19bEt
nk19cEt	rps6ka1
nk1bEt
nk1cEt
nk20aEt
nk22aEt
nk23aEt
nk23cEt
nk25aEt
nk26aEt
nk27aEt
nk2aEt
nk30aEt
nk37aEt
nk38BEt
nk3aEt	wbp2nl
nk3bEt
nk4aEt
nk4bEt
nk4cEt	camk2g1
nk55bEt
nk6aEt
nkhspggff19bTg

Transgenics

That Utilize Et(hsp70l:GFP-GAL4FF) Construct

Fish	Affected Genomic Regions	Phenotype	Gene Expression
camk2g1^nk4cEt; nkuasgfp1aTg	camk2g1		Fig. S5 from Asakawa et al., 2008
ctbp2a^nk17bEt; nkuasgfp1aTg	ctbp2a		Fig. S5 from Asakawa et al., 2008
gria4b^nk10bEt; nkuasgfp1aTg	gria4b		Fig. S5 from Asakawa et al., 2008
nk10cEt; nkuasgfp1aTg			Fig. S5 from Asakawa et al., 2008
nk14aEt; nkuasgfp1aTg			Fig. S5 from Asakawa et al., 2008
nk14bEt; nkuasgfp1aTg			Fig. S5 from Asakawa et al., 2008
nk14cEt; nkuasgfp1aTg			Fig. S5 from Asakawa et al., 2008
nk17cEt; nkuasgfp1aTg			Fig. S5 from Asakawa et al., 2008
nk19bEt; nkuasgfp1aTg			Fig. S5 from Asakawa et al., 2008
nk1bEt			Fig. 1 from Asakawa et al., 2008
nk1bEt; nkuasgfp1aTg			2 figures from Asakawa et al., 2008
nk1bEt; nkuasrfp1aTg			Fig. 1 from Asakawa et al., 2008
nk1cEt; nkuasgfp1aTg			Fig. S5 from Asakawa et al., 2008
nk20aEt; nkuasgfp1aTg			2 figures from 2 publications
nk20aEt; nkuasgfp1aTg + MO2-invs		2 figures from Fukui et al., 2012
nk20aEt; nkuasgfp1aTg + MO2-nek8		Fig. 2 from Fukui et al., 2012
nk20aEt; nkuasgfp1aTg + MO3-nek8		2 figures from Fukui et al., 2012
nk22aEt; nkuasgfp1aTg			Fig. S5 from Asakawa et al., 2008
nk23aEt; nkuasgfp1aTg			Fig. S5 from Asakawa et al., 2008
nk23cEt; nkuasgfp1aTg			Fig. S5 from Asakawa et al., 2008
nk25aEt; nkuasgfp1aTg			Fig. S5 from Asakawa et al., 2008
nk26aEt; nkuasgfp1aTg			Fig. S5 from Asakawa et al., 2008
nk27aEt; nkGCaMPHS4aTg
nk27aEt; nkUAS:Gtuba2Tg
nk27aEt; nkuasgfp1aTg			3 figures from Asakawa et al., 2008
nk27aEt; nkuastetxlc13aTg
nk27aEt; nkuastetxlccfp6aTg		Fig. 3 from Asakawa et al., 2008	2 figures from Asakawa et al., 2008
nk2aEt; nkuasgfp1aTg			Fig. S5 from Asakawa et al., 2008
nk30aEt; nkuasgfp1aTg			Fig. S5 from Asakawa et al., 2008
nk37aEt; nkuasgfp1aTg			Fig. S5 from Asakawa et al., 2008
nk3bEt; nkuasgfp1aTg			Fig. S5 from Asakawa et al., 2008
nk4aEt; nkuasgfp1aTg			Fig. S5 from Asakawa et al., 2008
nk4bEt; nkuasgfp1aTg			Fig. S5 from Asakawa et al., 2008
nk6aEt; nkuasgfp1aTg			Fig. S5 from Asakawa et al., 2008
nkhspggff19bTg; nkuasgfp1aTg			Fig. 2 from Reiten et al., 2017
rps6ka1^nk19cEt; nkuasgfp1aTg	rps6ka1		Fig. S5 from Asakawa et al., 2008
skib^nk15aEt	skib	text only from Asakawa et al., 2008	Fig. 2 from Asakawa et al., 2008
skib^nk15aEt; nkuasgfp1aTg	skib		3 figures from Asakawa et al., 2008
skib^nk15aEt; nkuasrfp1aTg	skib		Fig. 2 from Asakawa et al., 2008
skib^nk15aEt; nkuastetxlccfp6aTg	skib	text only from Asakawa et al., 2008
wbp2nl^nk3aEt; nkuasgfp1aTg	wbp2nl		Fig. S5 from Asakawa et al., 2008
zgc:92242^nk17aEt; nkuasgfp1aTg	zgc:92242		Fig. S5 from Asakawa et al., 2008

Sequence Information

Other Construct Pages

None

Citations