CRISPR

CRISPR6-egr3

ID

ZDB-CRISPR-241231-3

Name

CRISPR6-egr3

Previous Names

None

Target

egr3 (1)

Sequence

5' - GGCAAGGATGAGGACGTGGC - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

The PAM site was "CGG" at the 3' end.

Genome Resources

None

Target Location

Genome Build: GRCz11Chromosome: 8

Constructs

No data available

Genomic Features

Genomic Feature	Affected Genomic Regions
bns576Pt	egr3
bns577	egr3

Expression

Gene expression in Wild Types + CRISPR6-egr3

No data available

Phenotype

Phenotype resulting from CRISPR6-egr3

No data available

Phenotype of all Fish created by or utilizing CRISPR6-egr3

Phenotype	Fish	Conditions	Figures
atrioventricular canal endocardium spp1 expression decreased amount, abnormal	egr3^{bns577/bns577}	standard conditions	Fig. 3. from da Silva et al., 2024
atrioventricular canal endocardium nr4a2b expression decreased amount, abnormal	egr3^{bns577/bns577}	standard conditions	Fig. 3. from da Silva et al., 2024
atrioventricular canal endocardium EGFP expression increased distribution, abnormal	egr3^bns576Pt/+; bns193Tg; nkuasgfp1aTg; s896Tg	mechanical stress	Fig. 5. from da Silva et al., 2024
atrioventricular canal endothelial cell migration decreased process quality, abnormal	egr3^bns577/+; egr3^bns576Pt/+; is4Tg; nkuasgfp1aTg	standard conditions	Fig. 3. from da Silva et al., 2024
atrioventricular valve formation decreased process quality, abnormal	egr3^bns577/+; egr3^bns576Pt/+; is4Tg; nkuasgfp1aTg	standard conditions	Fig. 3. from da Silva et al., 2024
atrioventricular canal endothelial cell migration decreased process quality, abnormal	egr3^bns577/+; egr3^bns576Pt/+; rk8Tg	standard conditions	Fig. 4. from da Silva et al., 2024
atrioventricular valve formation decreased process quality, abnormal	egr3^bns577/+; egr3^bns576Pt/+; rk8Tg	standard conditions	Fig. 4. from da Silva et al., 2024
heart lacks all parts of type atrioventricular valve, abnormal	egr3^bns577/+; egr3^bns661Tg/+; bns193Tg; s896Tg; s898Tg	standard conditions	Fig. 2. from da Silva et al., 2024
atrioventricular valve formation decreased process quality, abnormal	egr3^bns577/+; egr3^bns661Tg/+; bns193Tg; s896Tg; s898Tg	standard conditions	Fig. 2. from da Silva et al., 2024
ventriculo bulbo valve formation decreased process quality, abnormal	egr3^{bns577/bns577}; bns193Tg; s843Tg	standard conditions	Fig. 1. from da Silva et al., 2024
heart lacks all parts of type atrioventricular valve, abnormal	egr3^{bns577/bns577}; bns193Tg; s843Tg	standard conditions	Fig. 1. from da Silva et al., 2024
atrioventricular valve formation decreased process quality, abnormal	egr3^{bns577/bns577}; bns193Tg; s843Tg	standard conditions	Fig. 1. from da Silva et al., 2024
heart lacks all parts of type ventriculo bulbo valve, abnormal	egr3^{bns577/bns577}; bns193Tg; s843Tg	standard conditions	Fig. 1. from da Silva et al., 2024
heart edematous, abnormal	egr3^{bns577/bns577}; bns193Tg; s843Tg	standard conditions	Fig. 1. from da Silva et al., 2024
atrioventricular canal endothelial cell migration process quality, ameliorated	egr3^bns577/+; egr3^bns576Pt/+; bns719Tg; rk8Tg	standard conditions	Fig. 4. from da Silva et al., 2024
atrioventricular valve formation process quality, ameliorated	egr3^bns577/+; egr3^bns576Pt/+; bns719Tg; rk8Tg	standard conditions	Fig. 4. from da Silva et al., 2024

Citations

Include unpublished citations

da Silva, A.R., Gunawan, F., Boezio, G.L.M., Faure, E., Théron, A., Avierinos, J.F., Lim, S., Jha, S.G., Ramadass, R., Guenther, S., Looso, M., Zaffran, S., Juan, T., Stainier, D.Y.R. (2024) egr3 is a mechanosensitive transcription factor gene required for cardiac valve morphogenesis. Science advances. 10:eadl0633

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