CRISPR

CRISPR1-snai1b

ID

ZDB-CRISPR-220427-1

Name

CRISPR1-snai1b

Previous Names

None

Target

snai1b (1)

Sequence

5' - GTCTATAAGTGGCGCAG - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

Targeting the proximal promoter of snai1b.

Genome Resources

None

Target Location

Constructs

No data available

Genomic Features

Genomic Feature	Affected Genomic Regions
bns351	snai1b

Expression

Gene expression in Wild Types + CRISPR1-snai1b

No data available

Phenotype

Phenotype resulting from CRISPR1-snai1b

No data available

Phenotype of all Fish created by or utilizing CRISPR1-snai1b

Phenotype	Fish	Conditions	Figures
cardiac muscle cell separated from myocardium, abnormal	snai1b^{bns351/bns351}; bns193Tg	standard conditions	Figure 1 supplement 1 from Gentile et al., 2021
heart trabecula formation decreased process quality, abnormal	snai1b^{bns351/bns351}; bns193Tg	standard conditions	Figure 1 supplement 4 from Gentile et al., 2021
cardiac muscle cell protruding out of myocardium, abnormal	snai1b^{bns351/bns351}; bns193Tg	standard conditions	Figure 1 from Gentile et al., 2021
cardiac muscle cell protruding out of myocardium, abnormal	snai1b^{bns351/bns351}; bns193Tg	standard conditions	Figure 1 supplement 1 from Gentile et al., 2021
trabecular layer of ventricle has fewer parts of type cardiac muscle cell, abnormal	snai1b^{bns351/bns351}; bns193Tg	standard conditions	Figure 1 supplement 4 from Gentile et al., 2021
heart desmb expression increased amount, abnormal	snai1b^{bns351/bns351}; bns193Tg	standard conditions	Figure 3 from Gentile et al., 2021
heart looping decreased process quality, abnormal	snai1b^{bns351/bns351}; bns193Tg	standard conditions	Figure 1 supplement 1 from Gentile et al., 2021
cardiac muscle cell basal part of cell Ab4-ctnna labeling increased amount, abnormal	snai1b^{bns351/bns351}; s883Tg	standard conditions	Figure 2 from Gentile et al., 2021
cardiac muscle cell basal part of cell ab1-myl-ser20 labeling increased amount, abnormal	snai1b^{bns351/bns351}; s883Tg	standard conditions	Figure 2 from Gentile et al., 2021
cardiac muscle cell cell-cell junction ab1-desm labeling decreased amount, abnormal	snai1b^{bns351/bns351}; s883Tg	standard conditions	Figure 3 from Gentile et al., 2021
cardiac muscle cell apical part of cell decreased area, abnormal	snai1b^{bns351/bns351}; s883Tg	standard conditions	Figure 1 supplement 5 from Gentile et al., 2021
cardiac ventricle decreased volume, abnormal	snai1b^{bns351/bns351}; s883Tg	standard conditions	Figure 1 supplement 5 from Gentile et al., 2021
cardiac muscle cell regulation of cell-cell adhesion mediated by cadherin decreased process quality, abnormal	snai1b^{bns351/bns351}; s883Tg	standard conditions	Figure 2 from Gentile et al., 2021
heart contraction decreased frequency, abnormal	snai1b^{bns351/bns351}; s883Tg	standard conditions	Figure 1 supplement 5 from Gentile et al., 2021
cardiac muscle cell basal part of cell ab1-desm labeling increased amount, abnormal	snai1b^{bns351/bns351}; s883Tg	standard conditions	Figure 3 from Gentile et al., 2021
cardiac muscle cell cell-cell junction Ab12-cdh2 labeling decreased amount, abnormal	snai1b^{bns351/bns351}; s883Tg	standard conditions	Figure 2 from Gentile et al., 2021
cardiac muscle cell protruding out of myocardium, ameliorated	snai1b^{bns351/bns351}; bns193Tg + MO1-tnnt2a	standard conditions	Figure 1 from Gentile et al., 2021
heart contraction arrested, abnormal	snai1b^{bns351/bns351}; bns193Tg + MO1-tnnt2a	standard conditions	Figure 1 from Gentile et al., 2021
cardiac muscle cell protruding out of myocardium, ameliorated	snai1b^{bns351/bns351}; bns555Tg	standard conditions	Figure 1 supplement 1 from Gentile et al., 2021

Citations