CRISPR

CRISPR4-chd7

ID

ZDB-CRISPR-220331-16

Name

CRISPR4-chd7

Previous Names

None

Target

chd7 (1)

Sequence

5' - TGTATTCCTGCTGTGCACAA - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

None

Genome Resources

None

Target Location

Constructs

No data available

Genomic Features

Genomic Feature	Affected Genomic Regions
iaf17	chd7

Expression

Gene expression in Wild Types + CRISPR4-chd7

No data available

Phenotype

Phenotype resulting from CRISPR4-chd7

No data available

Phenotype of all Fish created by or utilizing CRISPR4-chd7

Phenotype	Fish	Conditions	Figures
ventral mandibular arch decreased length, abnormal	chd7^iaf17/iaf17	standard conditions	Fig. 1 from Breuer et al., 2024
whole organism runx2b expression decreased amount, abnormal	chd7^iaf17/iaf17	standard conditions	Fig. 3 from Breuer et al., 2024
parasphenoid bone mineralization decreased process quality, abnormal	chd7^iaf17/iaf17	standard conditions	Fig. 1 from Breuer et al., 2024
supraneural decreased thickness, abnormal	chd7^iaf17/iaf17	standard conditions	Fig. 2 from Breuer et al., 2024
whole organism htr2b expression decreased amount, abnormal	chd7^iaf17/iaf17	standard conditions	Fig. 5 from Breuer et al., 2024
palatoquadrate cartilage ab1-col2a labeling decreased distribution, abnormal	chd7^iaf17/iaf17	standard conditions	Fig. 4 from Breuer et al., 2024
Meckel's cartilage sox9a expression decreased distribution, abnormal	chd7^iaf17/iaf17	standard conditions	Fig. 4 from Breuer et al., 2024
vertebra bone mineralization decreased rate, abnormal	chd7^iaf17/iaf17	standard conditions	Fig. 1 from Breuer et al., 2024
basihyal cartilage sox9a expression absent, abnormal	chd7^iaf17/iaf17	standard conditions	Fig. 4 from Breuer et al., 2024
scale osteoblast activation quality, abnormal	chd7^iaf17/iaf17	standard conditions	Fig. 3 from Breuer et al., 2024
opercle bone mineralization decreased process quality, abnormal	chd7^iaf17/iaf17	standard conditions	Fig. 1 from Breuer et al., 2024
osteoblast Ab3-runx2 labeling decreased distribution, abnormal	chd7^iaf17/iaf17	standard conditions	Fig. 3 from Breuer et al., 2024
Meckel's cartilage ab1-col2a labeling decreased distribution, abnormal	chd7^iaf17/iaf17	standard conditions	Fig. 4 from Breuer et al., 2024
Weberian apparatus decreased size, abnormal	chd7^iaf17/iaf17	standard conditions	Fig. 2 from Breuer et al., 2024
ceratohyal cartilage ab4-h3 labeling increased distribution, abnormal	chd7^iaf17/iaf17	standard conditions	Fig. 4 from Breuer et al., 2024
Weberian apparatus decreased thickness, abnormal	chd7^iaf17/iaf17	standard conditions	Fig. 2 from Breuer et al., 2024
tripus decreased thickness, abnormal	chd7^iaf17/iaf17	standard conditions	Fig. 2 from Breuer et al., 2024
precaudal vertebra increased volume, abnormal	chd7^iaf17/iaf17	standard conditions	Fig. 2 from Breuer et al., 2024
cranium decreased angle to trunk, abnormal	chd7^iaf17/iaf17	standard conditions	Fig. 1 from Breuer et al., 2024
ceratohyal cartilage col2a1a expression decreased distribution, abnormal	chd7^iaf17/iaf17	standard conditions	Fig. 4 from Breuer et al., 2024
ceratohyal cartilage increased angle to ceratohyal cartilage, abnormal	chd7^iaf17/iaf17	standard conditions	Fig. 1 from Breuer et al., 2024
ceratobranchial cartilage ab4-h3 labeling increased distribution, abnormal	chd7^iaf17/iaf17	standard conditions	Fig. 4 from Breuer et al., 2024
whole organism col2a1a expression decreased amount, abnormal	chd7^iaf17/iaf17	standard conditions	Fig. 4 from Breuer et al., 2024
neural arch increased angle to centrum, abnormal	chd7^iaf17/iaf17	standard conditions	Fig. 2 from Breuer et al., 2024
head osteoblast activation quality, abnormal	chd7^iaf17/iaf17	standard conditions	Fig. 3 from Breuer et al., 2024
head osteoclast active, abnormal	chd7^iaf17/iaf17	standard conditions	Fig. 3 from Breuer et al., 2024
ventral mandibular arch increased angle to head, abnormal	chd7^iaf17/iaf17	standard conditions	Fig. 1 from Breuer et al., 2024
ceratohyal cartilage cell population proliferation increased process quality, abnormal	chd7^iaf17/iaf17	standard conditions	Fig. 4 from Breuer et al., 2024
parapophysis decreased size, abnormal	chd7^iaf17/iaf17	standard conditions	Fig. 2 from Breuer et al., 2024
parapophysis decreased thickness, abnormal	chd7^iaf17/iaf17	standard conditions	Fig. 2 from Breuer et al., 2024
basihyal cartilage col2a1a expression absent, abnormal	chd7^iaf17/iaf17	standard conditions	Fig. 4 from Breuer et al., 2024
ceratobranchial cartilage ab1-col2a labeling decreased distribution, abnormal	chd7^iaf17/iaf17	standard conditions	Fig. 4 from Breuer et al., 2024
intercalarium decreased size, abnormal	chd7^iaf17/iaf17	standard conditions	Fig. 2 from Breuer et al., 2024
Meckel's cartilage ab4-h3 labeling increased distribution, abnormal	chd7^iaf17/iaf17	standard conditions	Fig. 4 from Breuer et al., 2024
whole organism postna expression decreased amount, abnormal	chd7^iaf17/iaf17	standard conditions	Fig. 3 from Breuer et al., 2024
palatoquadrate cartilage decreased length, abnormal	chd7^iaf17/iaf17	standard conditions	Fig. 1 from Breuer et al., 2024
whole organism sp7 expression decreased amount, abnormal	chd7^iaf17/iaf17	standard conditions	Fig. 3 from Breuer et al., 2024
neural arch deformed, abnormal	chd7^iaf17/iaf17	standard conditions	Fig. 2 from Breuer et al., 2024
tripus decreased size, abnormal	chd7^iaf17/iaf17	standard conditions	Fig. 2 from Breuer et al., 2024
ceratobranchial cartilage cell population proliferation increased process quality, abnormal	chd7^iaf17/iaf17	standard conditions	Fig. 4 from Breuer et al., 2024
scale osteoclast active, abnormal	chd7^iaf17/iaf17	standard conditions	Fig. 3 from Breuer et al., 2024
supraneural decreased size, abnormal	chd7^iaf17/iaf17	standard conditions	Fig. 2 from Breuer et al., 2024
whole organism ctsk expression increased amount, abnormal	chd7^iaf17/iaf17	standard conditions	Fig. 3 from Breuer et al., 2024
intercalarium decreased thickness, abnormal	chd7^iaf17/iaf17	standard conditions	Fig. 2 from Breuer et al., 2024
ceratohyal cartilage sox9a expression decreased distribution, abnormal	chd7^iaf17/iaf17	standard conditions	Fig. 4 from Breuer et al., 2024
precaudal vertebra bone mineralization decreased process quality, abnormal	chd7^iaf17/iaf17	standard conditions	Fig. 2 from Breuer et al., 2024
Meckel's cartilage cell population proliferation increased process quality, abnormal	chd7^iaf17/iaf17	standard conditions	Fig. 4 from Breuer et al., 2024
ceratobranchial cartilage sox9a expression absent, abnormal	chd7^iaf17/iaf17	standard conditions	Fig. 4 from Breuer et al., 2024
whole organism runx2a expression decreased amount, abnormal	chd7^iaf17/iaf17	standard conditions	Fig. 3 from Breuer et al., 2024
Meckel's cartilage col2a1a expression decreased distribution, abnormal	chd7^iaf17/iaf17	standard conditions	Fig. 4 from Breuer et al., 2024
ceratohyal cartilage ab1-col2a labeling decreased distribution, abnormal	chd7^iaf17/iaf17	standard conditions	Fig. 4 from Breuer et al., 2024
ceratobranchial cartilage col2a1a expression absent, abnormal	chd7^iaf17/iaf17	standard conditions	Fig. 4 from Breuer et al., 2024
precaudal vertebra chondrocyte decreased amount, abnormal	chd7^iaf17/iaf17	standard conditions	Fig. 4 from Breuer et al., 2024
vertebra increased volume, abnormal	chd7^iaf17/iaf17	standard conditions	Fig. 2 from Breuer et al., 2024
Weberian apparatus chondrocyte decreased amount, abnormal	chd7^iaf17/iaf17	standard conditions	Fig. 4 from Breuer et al., 2024
vertebra increased mass density, abnormal	chd7^iaf17/iaf17	standard conditions	Fig. 2 from Breuer et al., 2024

Citations