CRISPR

CRISPR1-prpf31

ID
ZDB-CRISPR-211101-1
Name
CRISPR1-prpf31
Previous Names
None
Target
Sequence
5' - TGGAGGACTGGAGGACATCCCGG - 3'
ZFIN BLAST NCBI BLAST Ensembl UCSC BLAT
Disclaimer
Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.
Note
None
Genome Resources
None
Target Location
Constructs
No data available
Genomic Features
Genomic Feature Affected Genomic Regions
hzu12 prpf31
Expression
Gene expression in Wild Types + CRISPR1-prpf31
No data available
Phenotype
Phenotype resulting from CRISPR1-prpf31
No data available
Phenotype of all Fish created by or utilizing CRISPR1-prpf31
Phenotype Fish Conditions Figures
retina ccnd1 expression increased amount, abnormal prpf31hzu12/hzu12 standard conditions Figure 2 with image from Li et al., 2021
whole organism baxa expression increased amount, abnormal prpf31hzu12/hzu12 standard conditions Figure 3 with image from Li et al., 2021
retina mitotic spindle morphology, abnormal prpf31hzu12/hzu12 standard conditions Figure 4 with image from Li et al., 2021
whole organism dead, abnormal prpf31hzu12/hzu12 standard conditions text only from Li et al., 2021
head rad51b expression decreased amount, abnormal prpf31hzu12/hzu12 standard conditions Figure 7 with image from Li et al., 2021
retina layer formation disrupted, abnormal prpf31hzu12/hzu12 standard conditions Figure S3 from Li et al., 2021
head rtel1 expression decreased amount, abnormal prpf31hzu12/hzu12 standard conditions Figure 7 with image from Li et al., 2021
glial cell differentiation disrupted, abnormal prpf31hzu12/hzu12 standard conditions Figure S4 from Li et al., 2021
whole organism ccng1 expression increased amount, abnormal prpf31hzu12/hzu12 standard conditions Figure 3 with image from Li et al., 2021
retina structure, abnormal prpf31hzu12/hzu12 standard conditions Figure S3 from Li et al., 2021
whole organism tp53 expression increased amount, abnormal prpf31hzu12/hzu12 standard conditions Figure 3 with image from Li et al., 2021
head rad17 expression decreased amount, abnormal prpf31hzu12/hzu12 standard conditions Figure 7 with image from Li et al., 2021
whole organism cdkn1a expression increased amount, abnormal prpf31hzu12/hzu12 standard conditions Figure 3 with image from Li et al., 2021
multi fate stem cell mitotic spindle morphology, abnormal prpf31hzu12/hzu12 standard conditions Figure 4 with image from Li et al., 2021
retina disorganized, abnormal prpf31hzu12/hzu12 standard conditions Figure S3 from Li et al., 2021
head rad9a expression decreased amount, abnormal prpf31hzu12/hzu12 standard conditions Figure 7 with image from Li et al., 2021
whole organism bbc3 expression increased amount, abnormal prpf31hzu12/hzu12 standard conditions Figure 3 with image from Li et al., 2021
retina mitotic M phase temporally extended, abnormal prpf31hzu12/hzu12 standard conditions Figure 4 with image from Li et al., 2021
whole organism mdm2 expression increased amount, abnormal prpf31hzu12/hzu12 standard conditions Figure 3 with image from Li et al., 2021
retina Ab11-sox2 labeling increased amount, abnormal prpf31hzu12/hzu12 standard conditions Figure 3 with image from Li et al., 2021
eye photoreceptor cell zpr-3 labeling decreased amount, abnormal prpf31hzu12/hzu12 standard conditions Figure 2 with image from Li et al., 2021
brain apoptotic process increased occurrence, abnormal prpf31hzu12/hzu12 standard conditions Figure 3 with image from Li et al., 2021
head Ab1-mlh1 labeling decreased amount, abnormal prpf31hzu12/hzu12 standard conditions Figure 7 with image from Li et al., 2021
retina mitotic cell cycle process quality, abnormal prpf31hzu12/hzu12 standard conditions Figure 4 with image from Li et al., 2021
eye decreased size, abnormal prpf31hzu12/hzu12 standard conditions Figure 1 with image from Li et al., 2021
whole organism decreased length, abnormal prpf31hzu12/hzu12 standard conditions Figure 1 with image from Li et al., 2021
retina vsx2 expression increased amount, abnormal prpf31hzu12/hzu12 standard conditions Figure 2 with image from Li et al., 2021
retina has fewer parts of type neuron, abnormal prpf31hzu12/hzu12 standard conditions Figure 2 with image from Li et al., 2021
head Ab1-prpf31 labeling decreased amount, abnormal prpf31hzu12/hzu12 standard conditions Figure 1 with image,  Figure 5 with image from Li et al., 2021
head sfi1 expression decreased amount, abnormal prpf31hzu12/hzu12 standard conditions Figure 7 with image from Li et al., 2021
head prpf31 expression decreased amount, abnormal prpf31hzu12/hzu12 standard conditions Figure 1 with image from Li et al., 2021
head ab13-tp53 labeling increased amount, abnormal prpf31hzu12/hzu12 standard conditions Figure 5 with image from Li et al., 2021
retina ab2-h2afx labeling increased amount, abnormal prpf31hzu12/hzu12 standard conditions Figure 5 with image from Li et al., 2021
neural retina development disrupted, abnormal prpf31hzu12/hzu12 standard conditions Figure 2 with image,  Figure 3 with image,  Figure S4 from Li et al., 2021
whole organism casp8 expression increased amount, abnormal prpf31hzu12/hzu12 standard conditions Figure 3 with image from Li et al., 2021
regulation of alternative mRNA splicing, via spliceosome disrupted, abnormal prpf31hzu12/hzu12 standard conditions Figure 5 with image,  Figure 7 with image from Li et al., 2021
whole organism bcl2a expression increased amount, abnormal prpf31hzu12/hzu12 standard conditions Figure 3 with image from Li et al., 2021
retina multi fate stem cell Ab11-sox2 labeling spatial pattern, abnormal prpf31hzu12/hzu12 standard conditions Figure 2 with image from Li et al., 2021
retina chromatin structure, abnormal prpf31hzu12/hzu12 standard conditions Figure S7 from Li et al., 2021
retina atoh7 expression decreased amount, abnormal prpf31hzu12/hzu12 standard conditions Figure 2 with image from Li et al., 2021
whole organism increased curvature, abnormal prpf31hzu12/hzu12 standard conditions Figure 1 with image from Li et al., 2021
retina crx expression decreased amount, abnormal prpf31hzu12/hzu12 standard conditions Figure 2 with image from Li et al., 2021
spinal cord apoptotic process increased occurrence, abnormal prpf31hzu12/hzu12 standard conditions Figure 3 with image from Li et al., 2021
head Ab1-rtel1 labeling decreased amount, abnormal prpf31hzu12/hzu12 standard conditions Figure 7 with image from Li et al., 2021
head ska1 expression decreased amount, abnormal prpf31hzu12/hzu12 standard conditions Figure 7 with image from Li et al., 2021
retina neurod1 expression decreased amount, abnormal prpf31hzu12/hzu12 standard conditions Figure 2 with image from Li et al., 2021
whole organism gadd45ba expression increased amount, abnormal prpf31hzu12/hzu12 standard conditions Figure 3 with image from Li et al., 2021
head mlh1 expression decreased amount, abnormal prpf31hzu12/hzu12 standard conditions Figure 7 with image from Li et al., 2021
retina mitotic cell cycle arrested, abnormal prpf31hzu12/hzu12 standard conditions Figure 4 with image from Li et al., 2021
retina apoptotic process increased occurrence, abnormal prpf31hzu12/hzu12 standard conditions Figure 3 with image,  Figure S7 from Li et al., 2021
retina site of DNA damage increased amount, abnormal prpf31hzu12/hzu12 standard conditions Figure 5 with image from Li et al., 2021
retina nucleus condensed, abnormal prpf31hzu12/hzu12 standard conditions Figure S3 from Li et al., 2021
head lrrcc1 expression decreased amount, abnormal prpf31hzu12/hzu12 standard conditions Figure 7 with image from Li et al., 2021
head ab2-h2afx labeling increased amount, abnormal prpf31hzu12/hzu12 standard conditions Figure 5 with image from Li et al., 2021
retina has extra parts of type multi fate stem cell, abnormal prpf31hzu12/hzu12 standard conditions Figure 2 with image,  Figure 3 with image from Li et al., 2021
retinal inner nuclear layer Ab6-isl1 labeling decreased amount, abnormal prpf31hzu12/hzu12 standard conditions Figure 2 with image from Li et al., 2021
head decreased size, abnormal prpf31hzu12/hzu12 standard conditions Figure 1 with image from Li et al., 2021
head kifc1 expression decreased amount, abnormal prpf31hzu12/hzu12 standard conditions Figure 7 with image from Li et al., 2021
retina Ab6-isl1 labeling decreased amount, abnormal prpf31hzu12/hzu12 standard conditions Figure 3 with image from Li et al., 2021
retina multi fate stem cell Ab11-sox2 labeling increased amount, abnormal prpf31hzu12/hzu12 standard conditions Figure 2 with image from Li et al., 2021
eye photoreceptor cell zpr-1 labeling decreased amount, abnormal prpf31hzu12/hzu12 standard conditions Figure 2 with image from Li et al., 2021
retina neuron EGFP expression decreased amount, abnormal prpf31hzu12/hzu12; knu3Tg standard conditions Figure 2 with image from Li et al., 2021
retina neuron EGFP expression decreased amount, abnormal prpf31hzu12/hzu12; nl1Tg standard conditions Figure 2 with image from Li et al., 2021
neural retina development disrupted, abnormal prpf31hzu12/hzu12; tp53ihb135/ihb135 standard conditions Figure 3 with image from Li et al., 2021
retina has extra parts of type multi fate stem cell, abnormal prpf31hzu12/hzu12; tp53ihb135/ihb135 standard conditions Figure 3 with image from Li et al., 2021
retina Ab6-isl1 labeling decreased amount, abnormal prpf31hzu12/hzu12; tp53ihb135/ihb135 standard conditions Figure 3 with image from Li et al., 2021
retina apoptotic process occurrence, ameliorated prpf31hzu12/hzu12; tp53ihb135/ihb135 standard conditions Figure 3 with image from Li et al., 2021
retina Ab11-sox2 labeling increased amount, abnormal prpf31hzu12/hzu12; tp53ihb135/ihb135 standard conditions Figure 3 with image from Li et al., 2021
Citations
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