CRISPR

CRISPR1-stab2

ID

ZDB-CRISPR-200224-2

Name

CRISPR1-stab2

Previous Names

None

Target

stab2 (1)

Sequence

5' - CACACACTCCTCAAGCAC - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

None

Genome Resources

None

Target Location

Constructs

No data available

Genomic Features

Genomic Feature	Affected Genomic Regions
ibl2	stab2

Expression

Gene expression in Wild Types + CRISPR1-stab2

No data available

Phenotype

Phenotype resulting from CRISPR1-stab2

No data available

Phenotype of all Fish created by or utilizing CRISPR1-stab2

Phenotype	Fish	Conditions	Figures
hepatic sinusoid import into cell decreased occurrence, abnormal	stab2^ibl2/ibl2	chemical treatment by injection: carbon black nanoparticle, chemical treatment by injection: lipid micelle	Fig. 3 , Fig. 4 from Campbell et al., 2018
hepatic sinusoid carbon black nanoparticle decreased amount, abnormal	stab2^ibl2/ibl2	chemical treatment by injection: carbon black nanoparticle, chemical treatment by injection: lipid micelle	Fig. 3 , Fig. 4 from Campbell et al., 2018
post-vent region stab2 expression decreased amount, abnormal	stab2^ibl2/ibl2	control	Fig. S10 from Campbell et al., 2018
scavenger receptor activity disrupted, abnormal	stab2^ibl2/ibl2	standard conditions	Fig. 3 from Arias-Alpizar et al., 2021
trunk stab2 expression decreased amount, abnormal	stab2^ibl2/ibl2	control	Fig. S10 from Campbell et al., 2018
blood vessel endothelial cell receptor-mediated endocytosis involved in cholesterol transport decreased process quality, abnormal	stab2^ibl2/ibl2 (AB/TL)	control	Fig. 1 from Verwilligen et al., 2022
caudal vein length, ameliorated	stab2^ibl2/ibl2; s843Tg	chemical treatment by injection: clodronic acid, chemical treatment by injection: carbon black nanoparticle, chemical treatment by injection: lipid micelle	Fig. 5 from Campbell et al., 2018
caudal vein morphology, ameliorated	stab2^ibl2/ibl2; s843Tg	chemical treatment by injection: clodronic acid, chemical treatment by injection: carbon black nanoparticle, chemical treatment by injection: lipid micelle	Fig. 5 from Campbell et al., 2018
whole organism semi-viable, abnormal	stab1^ibl3/ibl3; stab2^ibl2/ibl2	standard conditions	Fig. 1 from Arias-Alpizar et al., 2021
kidney decreased functionality, abnormal	stab1^ibl3/ibl3; stab2^ibl2/ibl2	standard conditions	Fig. 1 from Arias-Alpizar et al., 2021
scavenger receptor activity disrupted, abnormal	stab1^ibl3/ibl3; stab2^ibl2/ibl2	standard conditions	Fig. 2 , Fig. 3 from Arias-Alpizar et al., 2021
whole organism ldlra expression increased amount, abnormal	stab1^ibl3/ibl3; stab2^ibl2/ibl2 (AB/TL)	high cholesterol	Fig. 4 from Verwilligen et al., 2022
whole organism cd36 expression decreased amount, abnormal	stab1^ibl3/ibl3; stab2^ibl2/ibl2 (AB/TL)	high cholesterol	Fig. 4 from Verwilligen et al., 2022
blood vessel endothelial cell receptor-mediated endocytosis involved in cholesterol transport process quality, exacerbated	stab1^ibl3/ibl3; stab2^ibl2/ibl2 (AB/TL)	control	Fig. 1 from Verwilligen et al., 2022
whole organism scarb1 expression decreased amount, abnormal	stab1^ibl3/ibl3; stab2^ibl2/ibl2 (AB/TL)	high cholesterol	Fig. 4 from Verwilligen et al., 2022
whole organism marco expression increased amount, abnormal	stab1^ibl3/ibl3; stab2^ibl2/ibl2 (AB/TL)	standard conditions	Fig. 3 from Verwilligen et al., 2022
blood vessel endothelial cell low-density lipoprotein particle clearance decreased process quality, abnormal	stab1^ibl3/ibl3; stab2^ibl2/ibl2; gl23Tg (AB/TL)	control	Fig. 2 from Verwilligen et al., 2022
blood vessel endothelial cell oxidised low-density lipoprotein particle clearance decreased process quality, abnormal	stab1^ibl3/ibl3; stab2^ibl2/ibl2; gl23Tg (AB/TL)	control	Fig. 2 from Verwilligen et al., 2022

Citations