CRISPR

CRISPR6-tnnt2a

ID

ZDB-CRISPR-190522-3

Name

CRISPR6-tnnt2a

Previous Names

None

Target

tnnt2a (1)

Sequence

5' - GGGAGCGACAGAAGCGTT - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

None

Genome Resources

None

Target Location

Constructs

No data available

Genomic Features

Genomic Feature	Affected Genomic Regions
fdu108	tnnt2a

Expression

Gene expression in Wild Types + CRISPR6-tnnt2a

No data available

Phenotype

Phenotype resulting from CRISPR6-tnnt2a

No data available

Phenotype of all Fish created by or utilizing CRISPR6-tnnt2a

Phenotype	Fish	Conditions	Figures
heart muscle contraction arrested, abnormal	tnnt2a^{fdu108/fdu108}; fdu107Tg	standard conditions	Fig. 1. from Liu et al., 2019
pericardium edematous, abnormal	tnnt2a^{fdu108/fdu108}; twu34Tg	standard conditions	Fig. 1. from Liu et al., 2019
heart contraction arrested, abnormal	tnnt2a^{fdu108/fdu108}; twu34Tg	standard conditions	Fig. 1. from Liu et al., 2019
atrium distended, abnormal	tnnt2a^{fdu108/fdu108}; twu34Tg	standard conditions	Fig. 1. from Liu et al., 2019
cardiac ventricle distended, abnormal	tnnt2a^{fdu108/fdu108}; twu34Tg	standard conditions	Fig. 1. from Liu et al., 2019
heart tnnt2a expression amount, ameliorated	tnnt2a^{fdu108/fdu108}; fdu106Tg	chemical treatment by environment: doxycycline	Fig. 2. , Fig. 3. from Liu et al., 2019
heart myl7 expression increased amount, abnormal	tnnt2a^{fdu108/fdu108}; fdu106Tg	standard conditions	Fig. 3. from Liu et al., 2019
blood circulation decreased rate, abnormal	tnnt2a^{fdu108/fdu108}; fdu106Tg	standard conditions	Fig. 2. from Liu et al., 2019
blood circulation decreased rate, abnormal	tnnt2a^{fdu108/fdu108}; fdu106Tg	chemical treatment by environment: doxycycline	Fig. 2. from Liu et al., 2019
heart bmp2b expression decreased amount, abnormal	tnnt2a^{fdu108/fdu108}; fdu106Tg	standard conditions	Fig. 3. from Liu et al., 2019
heart tpm4a expression increased amount, abnormal	tnnt2a^{fdu108/fdu108}; fdu106Tg	standard conditions	Fig. 3. from Liu et al., 2019
pericardium edematous, abnormal	tnnt2a^{fdu108/fdu108}; fdu106Tg	standard conditions	Fig. 2. from Liu et al., 2019
heart tnni1b expression amount, ameliorated	tnnt2a^{fdu108/fdu108}; fdu106Tg	chemical treatment by environment: doxycycline	Fig. 3. from Liu et al., 2019
heart tbx20 expression increased amount, abnormal	tnnt2a^{fdu108/fdu108}; fdu106Tg	chemical treatment by environment: doxycycline	Fig. 3. from Liu et al., 2019
heart contraction arrested, abnormal	tnnt2a^{fdu108/fdu108}; fdu106Tg	standard conditions	Fig. 2. , Fig. 4. from Liu et al., 2019
atrium distended, abnormal	tnnt2a^{fdu108/fdu108}; fdu106Tg	standard conditions	Fig. 2. from Liu et al., 2019
heart tnnc1a expression decreased amount, abnormal	tnnt2a^{fdu108/fdu108}; fdu106Tg	standard conditions	Fig. 3. from Liu et al., 2019
cardiac ventricle distended, ameliorated	tnnt2a^{fdu108/fdu108}; fdu106Tg	chemical treatment by environment: doxycycline	Fig. 2. from Liu et al., 2019
atrium distended, ameliorated	tnnt2a^{fdu108/fdu108}; fdu106Tg	chemical treatment by environment: doxycycline	Fig. 2. from Liu et al., 2019
heart contraction rate, ameliorated	tnnt2a^{fdu108/fdu108}; fdu106Tg	chemical treatment by environment: doxycycline	Fig. 2. from Liu et al., 2019
heart contraction arrested, ameliorated	tnnt2a^{fdu108/fdu108}; fdu106Tg	chemical treatment by environment: doxycycline	Fig. 2. from Liu et al., 2019
heart actc1a expression amount, ameliorated	tnnt2a^{fdu108/fdu108}; fdu106Tg	chemical treatment by environment: doxycycline	Fig. 3. from Liu et al., 2019
heart myl7 expression amount, ameliorated	tnnt2a^{fdu108/fdu108}; fdu106Tg	chemical treatment by environment: doxycycline	Fig. 3. from Liu et al., 2019
bulbus arteriosus decreased diameter, abnormal	tnnt2a^{fdu108/fdu108}; fdu106Tg	chemical treatment by environment: doxycycline	Fig. 4. from Liu et al., 2019
heart gata5 expression increased amount, abnormal	tnnt2a^{fdu108/fdu108}; fdu106Tg	standard conditions	Fig. 3. from Liu et al., 2019
heart tnnt2a expression absent, abnormal	tnnt2a^{fdu108/fdu108}; fdu106Tg	standard conditions	Fig. 2. , Fig. 3. from Liu et al., 2019
bulbus arteriosus decreased contractility, abnormal	tnnt2a^{fdu108/fdu108}; fdu106Tg	chemical treatment by environment: doxycycline	Fig. 4. from Liu et al., 2019
cardiac ventricle distended, abnormal	tnnt2a^{fdu108/fdu108}; fdu106Tg	standard conditions	Fig. 2. from Liu et al., 2019
heart tpm4a expression increased amount, abnormal	tnnt2a^{fdu108/fdu108}; fdu106Tg	chemical treatment by environment: doxycycline	Fig. 3. from Liu et al., 2019
pericardium edematous, abnormal	tnnt2a^{fdu108/fdu108}; fdu106Tg	chemical treatment by environment: doxycycline	Fig. 2. from Liu et al., 2019
heart has2 expression decreased amount, abnormal	tnnt2a^{fdu108/fdu108}; fdu106Tg	chemical treatment by environment: doxycycline	Fig. 3. from Liu et al., 2019
heart contraction decreased rate, abnormal	tnnt2a^{fdu108/fdu108}; fdu106Tg	chemical treatment by environment: doxycycline	Fig. 4. from Liu et al., 2019
heart tbx2b expression decreased amount, abnormal	tnnt2a^{fdu108/fdu108}; fdu106Tg	standard conditions	Fig. 3. from Liu et al., 2019
heart actc1a expression increased amount, abnormal	tnnt2a^{fdu108/fdu108}; fdu106Tg	standard conditions	Fig. 3. from Liu et al., 2019
heart gata5 expression increased amount, abnormal	tnnt2a^{fdu108/fdu108}; fdu106Tg	chemical treatment by environment: doxycycline	Fig. 3. from Liu et al., 2019
heart gata4 expression increased amount, abnormal	tnnt2a^{fdu108/fdu108}; fdu106Tg	standard conditions	Fig. 3. from Liu et al., 2019
heart tnni1b expression increased amount, abnormal	tnnt2a^{fdu108/fdu108}; fdu106Tg	standard conditions	Fig. 3. from Liu et al., 2019
heart tnnc1a expression decreased amount, abnormal	tnnt2a^{fdu108/fdu108}; fdu106Tg	chemical treatment by environment: doxycycline	Fig. 3. from Liu et al., 2019
heart gata4 expression increased amount, abnormal	tnnt2a^{fdu108/fdu108}; fdu106Tg	chemical treatment by environment: doxycycline	Fig. 3. from Liu et al., 2019
heart bmp2b expression decreased amount, abnormal	tnnt2a^{fdu108/fdu108}; fdu106Tg	chemical treatment by environment: doxycycline	Fig. 3. from Liu et al., 2019
heart tbx2b expression decreased amount, abnormal	tnnt2a^{fdu108/fdu108}; fdu106Tg	chemical treatment by environment: doxycycline	Fig. 3. from Liu et al., 2019

Citations