CRISPR

CRISPR1-rfx4

ID

ZDB-CRISPR-180801-2

Name

CRISPR1-rfx4

Previous Names

None

Target

rfx4 (1)

Sequence

5' - TCTCAGGCGTCCTCATGCCC - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

None

Genome Resources

None

Target Location

Constructs

No data available

Genomic Features

Genomic Feature	Affected Genomic Regions
uw8013	rfx4
uw8110	rfx4

Expression

Gene expression in Wild Types + CRISPR1-rfx4

No data available

Phenotype

Phenotype resulting from CRISPR1-rfx4

No data available

Phenotype of all Fish created by or utilizing CRISPR1-rfx4

Phenotype	Fish	Conditions	Figures
hindbrain dorsal region wnt1 expression decreased amount, abnormal	rfx4^{uw8013/uw8013}	standard conditions	Fig. 6 from Sedykh et al., 2017
hindbrain dorsal region zic2a expression decreased amount, abnormal	rfx4^{uw8013/uw8013}	standard conditions	Fig. 3 from Sedykh et al., 2017
spinal cord zic2a expression decreased amount, abnormal	rfx4^{uw8013/uw8013}	standard conditions	Fig. 3 from Sedykh et al., 2017
roof plate cell disorganized, abnormal	rfx4^{uw8013/uw8013}	standard conditions	Fig. 5 from Sedykh et al., 2017
whole organism decreased life span, abnormal	rfx4^{uw8013/uw8013}	standard conditions	Fig. 2 from Sedykh et al., 2017
hindbrain dorsal region atoh1a expression decreased amount, abnormal	rfx4^{uw8013/uw8013}	standard conditions	Fig. 6 from Sedykh et al., 2017
floor plate foxa2 expression spatial pattern, abnormal	rfx4^{uw8013/uw8013}	standard conditions	Fig. 6 from Sedykh et al., 2017
trunk curved ventral, abnormal	rfx4^{uw8013/uw8013}	standard conditions	Fig. 2 from Sedykh et al., 2017
swim bladder uninflated, abnormal	rfx4^{uw8013/uw8013}	standard conditions	Fig. 2 from Sedykh et al., 2017
hindbrain dorsal region wnt3a expression decreased amount, abnormal	rfx4^{uw8013/uw8013}	standard conditions	Fig. 3 from Sedykh et al., 2017
floor plate spinal cord region shha expression decreased amount, abnormal	rfx4^{uw8013/uw8013}	standard conditions	Fig. 4 from Sedykh et al., 2017
roof plate cell amount, abnormal	rfx4^{uw8013/uw8013}	standard conditions	Fig. 5 from Sedykh et al., 2017
whole organism dead, abnormal	rfx4^{uw8013/uw8013}	standard conditions	Fig. 2 from Sedykh et al., 2017
floor plate spinal cord region ptch2 expression decreased amount, abnormal	rfx4^{uw8013/uw8013}	standard conditions	Fig. 4 from Sedykh et al., 2017
midbrain dorsal region wnt1 expression decreased amount, abnormal	rfx4^{uw8013/uw8013}	standard conditions	Fig. 6 from Sedykh et al., 2017
floor plate spinal cord region foxa2 expression decreased amount, abnormal	rfx4^{uw8013/uw8013}	standard conditions	Fig. 6 from Sedykh et al., 2017

Citations