CRISPR

CRISPR1-piezo1

ID

ZDB-CRISPR-170908-2

Name

CRISPR1-piezo1

Previous Names

None

Target

piezo1 (1)

Sequence

5' - GTAGCGAAATATGCAGGCTG - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

None

Genome Resources

None

Target Location

Constructs

No data available

Genomic Features

Genomic Feature	Affected Genomic Regions
ou2048	piezo1

Expression

Gene expression in Wild Types + CRISPR1-piezo1

No data available

Phenotype

Phenotype resulting from CRISPR1-piezo1

Phenotype	Fish	Figures
epidermis mitotic cell cycle decreased rate, abnormal	AB + CRISPR1-piezo1	Fig. 2 from Gudipaty et al., 2017

Phenotype of all Fish created by or utilizing CRISPR1-piezo1

Phenotype	Fish	Conditions	Figures
whole organism decreased length, abnormal	piezo1^{ou2048/ou2048}	standard conditions	FIGURE 3 from Ramli et al., 2024
vertebra bone mineralization decreased magnitude, abnormal	piezo1^{ou2048/ou2048}	standard conditions	FIGURE 4 , FIGURE 7 from Ramli et al., 2024
vertebral column bone tissue piezo1 expression decreased amount, abnormal	piezo1^{ou2048/ou2048}	standard conditions	FIGURE 2 from Ramli et al., 2024
vertebra osteoblast bglap expression decreased amount, abnormal	piezo1^{ou2048/ou2048}	standard conditions	FIGURE 5 from Ramli et al., 2024
vertebra bone tissue decreased mass density, abnormal	piezo1^{ou2048/ou2048}	standard conditions	FIGURE 4 , FIGURE 7 from Ramli et al., 2024
vertebral column bone tissue mislocalised, abnormal	piezo1^{ou2048/ou2048}	standard conditions	FIGURE 3 , FIGURE 7 from Ramli et al., 2024
vertebral column curved, abnormal	piezo1^{ou2048/ou2048}	standard conditions	FIGURE 3 , FIGURE 7 from Ramli et al., 2024
vertebra osteoblast sp7 expression decreased amount, abnormal	piezo1^{ou2048/ou2048}	standard conditions	FIGURE 5 from Ramli et al., 2024
epidermis mitotic cell cycle decreased rate, abnormal	AB + CRISPR1-piezo1	standard conditions	Fig. 2 from Gudipaty et al., 2017
vertebra bone mineralization magnitude, ameliorated	mstnb^{ou2052/ou2052}; piezo1^{ou2048/ou2048}	standard conditions	FIGURE 7 from Ramli et al., 2024
vertebra bone tissue normal mass density, ameliorated	mstnb^{ou2052/ou2052}; piezo1^{ou2048/ou2048}	standard conditions	FIGURE 7 from Ramli et al., 2024
vertebral column morphology, ameliorated	mstnb^{ou2052/ou2052}; piezo1^{ou2048/ou2048}	standard conditions	FIGURE 7 from Ramli et al., 2024
vertebral column bone tissue position, ameliorated	mstnb^{ou2052/ou2052}; piezo1^{ou2048/ou2048}	standard conditions	FIGURE 7 from Ramli et al., 2024

Citations