Molecular Probes for zebrafish currently available:
that recognize a variety of structures in zebrafish embryos are available from the library in Eugene.
Click here for a listing.
Antibodies can be ordered from the Zebrafish International Resource Center.
Additionally, we can be contacted at:
The Zebrafish International Resource Center
5274 University of Oregon
Eugene, OR 97403-5274
Two BAC libraries are currently available that have been constructed from
Tu strain DNA,
the same DNA currently be used for the Sanger
Center genome sequencing project.
- CHORI-211 BAC library:
The CHORI-211 BAC library was constructed by Chung
Li Shu and Kazutoyo Osoegawa in Pieter J. de Jong’s
laboratory at Children's Hospital Oakland Research
Institute. Genomic DNA was isolated from testis
of 6 males by Dr. Gerd-Joerg Rauch and partially
digested with a combination of EcoRI and EcoRI Methylase.
Size selected DNA was cloned into the pTARBAC2.1
vector between the EcoRI sites. The ligation products
were transformed into DH10B electro-competent cells
(BRL Life Technologies). The library has been arrayed
into 384-well microtiter dishes and also gridded
onto 6 22x22cm nylon high-density filters for screening
by probe hybridization. Each hybridization membrane
represents over 18,000 distinct zebrafish BAC clones,
stamped in duplicate. Library construction was supported
by sub-contracts from a grant awarded to Robert
Geisler at The Max-Planck-Institut für Entwicklungsbiologie,
Tübingen. For more information check: http://www.chori.org/bacpac/home.htm
- Daniokey BAC library:
The Daniokey BAC library, created by the Ronald
Plasterk Laboratory and Keygene N.V., is available
via the RZPD in
Berlin. The library is available as glycerol stocks,
filters, and PCR pools. It, too, was constructed
strain genomic DNA. A partial HindIII digest
resulted in 104,064 BAC clones with an average insert
size of ~175 kb. The library provides approximately
10x coverage of the zebrafish genome.
BAC libraries used in the Sanger project.
- BUSM1 PAC library. The BUSM1
PAC library was constructed by Chris
Amemiya while he was at Boston University School of Medicine. The library
was constructed using MboI partial digests of high molecular weight DNA from
blood cells of ~ 200 individuals (AB
strain) ligated to the BamHI sites of pCYPAC6 (GenBank AF133437). The
library is archived in 271 384-well dishes. High-density filters and PCR-screenable
pools for this library are available from the RZPD. In
addition to the RZPD, numerous other labs have replicas of this library, including:
Will Talbot, Steve Johnson, John Postlethwait, Len Zon, Marnie Halpern, Shuo
Lin, and Didier Stanier. Copies of the library also exist in Japan and Europe.
Special requests for pools for PCR screening and/or high density filters
can be made to Chris Amemiya (firstname.lastname@example.org).
The Zebrafish YAC
Library, constructed by the Massachusetts General Hospital in collaboration
with the MIT Center for Genome Research, is available from Research
Genetics. The average insert size for the library is 480 kb. With approximately
19,000 clones, the library represents five zebrafish genome equivalents. Inserts
were cloned into the EcoRI site of the pRML1 and pRML2 vector arms. J57D was
used as the yeast host strain. Ref: T.P. Zhong, K. Kaphingst, U.Akella, M.
Haldi, E.S. Lander and M. Fishman Genomics 48, 136-138 (1998).
A random-primed shield-stage cDNA library, cloned into lambda ZAPII, and amplified
from 6.5x106 independent recombinants is
Laboratory of Molecular Genetics
Building 6B, Room 4B-422
9000 Rockville Pike
Bethesda, MD 20892
fax: (301) 496-0243
libraries are unfortunately no longer available from David Grunwald or Bruce Appel.
If you know of any other sources, please contact us.