Research
General Information
ZIRC
ZFIN ID: ZDB-PUB-040907-1
       

Fast Release Clones: A High Throughput Expression Analysis

Thisse, B., Thisse, C.

DATE: 2004 SOURCE: ZFIN Direct Data Submission
REGISTERED AUTHORS: Thisse, Bernard, Thisse, Christine
KEYWORDS: Fast Release, Expression

PubMed: none    

ABSTRACT:

Summary
We perform a rapid large scale in situ hybridization screen to characterize genes expressed in a spatially regulated manner during zebrafish embryogenesis. cDNAs collected from ZGC and I.M.A.G.E. libraries are used as templates for the synthesis of digoxygenin labeled antisense RNA probes. These probes are subsequently used to analyze the expression pattern of the corresponding gene at different developmental stages (from gastrula to hatching). These data are deposited in the ZFIN database. This work provides hundreds of specific cell or tissue markers to analyze mutant phenotypes and to help identify candidates for mutant loci or downstream targets of regulatory genes. This project allows the description of zebrafish embryonic development in terms of gene expression and will eventually establish a "molecular anatomy" of the developing embryo.

Method:

Acknowledging this work:
All clones, information, and images used from this work should cite this summary. If you have any questions or comments on this project, please contact C and B Thisse.

ERRATA and NOTES:
The cDNA and in situ hybridizations for Fast Release clones (high throughput analysis) have not been double checked. Mistakes may occur. Please contact C and B Thisse if you detect anything wrong. PCR protocol available on the probe details page.