Gene
irx2a
- ID
- ZDB-GENE-040426-1446
- Name
- iroquois homeobox 2a
- Symbol
- irx2a Nomenclature History
- Previous Names
- Type
- protein_coding_gene
- Location
- Chr: 16 Mapping Details/Browsers
- Description
- Predicted to enable DNA-binding transcription factor activity, RNA polymerase II-specific and RNA polymerase II cis-regulatory region sequence-specific DNA binding activity. Acts upstream of or within pronephric nephron tubule epithelial cell differentiation and retina morphogenesis in camera-type eye. Predicted to be active in nucleus. Is expressed in several structures, including basal plate midbrain region; nervous system; otic vesicle; pectoral fin bud; and pronephric tubule. Orthologous to human IRX2 (iroquois homeobox 2).
- Genome Resources
- Note
- None
- Comparative Information
-
- All Expression Data
- 24 figures from 8 publications
- Cross-Species Comparison
- High Throughput Data
- Thisse Expression Data
-
- MGC:77209 (6 images)
- eu2449 (9 images)
Wild Type Expression Summary
Phenotype Summary
Mutations
| Allele | Type | Localization | Consequence | Mutagen | Supplier |
|---|---|---|---|---|---|
| el831 | Allele with one delins | Unknown | Unknown | CRISPR | |
| sa10776 | Allele with one point mutation | Unknown | Premature Stop | ENU | |
| sa17696 | Allele with one point mutation | Unknown | Splice Site | ENU | |
| sa19128 | Allele with one point mutation | Unknown | Premature Stop | ENU | |
| sa22736 | Allele with one point mutation | Unknown | Premature Stop | ENU |
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| Targeting Reagent | Created Alleles | Citations |
|---|---|---|
| CRISPR1-irx2a | Farmer et al., 2021 | |
| MO1-irx2a | N/A | Choy et al., 2010 |
| MO2-irx2a | N/A | (2) |
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Human Disease
Domain, Family, and Site Summary
Domain Details Per Protein
| Protein | Length | Homedomain-like superfamily | Homeobox, conserved site | Homeodomain | Iroquois-class homeodomain protein | KN homeodomain |
|---|---|---|---|---|---|---|
|
UniProtKB:Q7SZP0
|
432 |
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Interactions and Pathways
No data available
Plasmids
No data available
| Construct | Regulatory Region | Coding Sequence | Species | Tg Lines | Citations |
|---|---|---|---|---|---|
| Tg1(FRT-Xla.Actc1:DsRed-GAB-FRT,LOXP-irx2a-LOXP-gata2a:EGFP-5HS4) |
|
| 3 | (4) | |
| Tg2(FRT-Xla.Actc1:DsRed-GAB-FRT,LOXP-irx2a-LOXP-gata2a:EGFP-5HS4) |
|
| 3 | (4) |
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| Relationship | Marker Type | Marker | Accession Numbers | Citations |
|---|---|---|---|---|
| Contained in | Fosmid | ZFOS-267C11 | ZFIN Curated Data | |
| Contained in | Fosmid | ZFOS-1179H2 | ZFIN Curated Data | |
| Contains | SSLP | z7233 | Dildrop et al., 2004 | |
| Encodes | EST | eu2449 | Thisse et al., 2005 | |
| Encodes | cDNA | MGC:65872 | ZFIN Curated Data | |
| Encodes | cDNA | MGC:77209 | ZFIN Curated Data |
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| Type | Accession # | Sequence | Length (nt/aa) | Analysis |
|---|---|---|---|---|
| RNA | RefSeq:NM_201057 (1) | 2239 nt | ||
| Genomic | GenBank:CU658470 (2) | 45186 nt | ||
| Polypeptide | UniProtKB:Q7SZP0 (1) | 432 aa |
- Farmer, D.T., Patel, P., Choi, R., Liu, C.Y., Crump, J.G. (2021) Comprehensive series of Irx cluster mutants reveals diverse roles in facial cartilage development. Development (Cambridge, England). 148(16)
- Lush, M.E., Diaz, D.C., Koenecke, N., Baek, S., Boldt, H., St Peter, M.K., Gaitan-Escudero, T., Romero-Carvajal, A., Busch-Nentwich, E.M., Perera, A.G., Hall, K.E., Peak, A., Haug, J.S., Piotrowski, T. (2019) scRNA-Seq reveals distinct stem cell populations that drive hair cell regeneration after loss of Fgf and Notch signaling. eLIFE. 8:
- Marra, A.N., Cheng, C.N., Adeeb, B., Addiego, A., Wesselman, H.M., Chambers, B.E., Chambers, J.M., Wingert, R.A. (2019) Iroquois transcription factor irx2a is required for multiciliated and transporter cell fate decisions during zebrafish pronephros development. Scientific Reports. 9:6454
- Elkon, R., Milon, B., Morrison, L., Shah, M., Vijayakumar, S., Racherla, M., Leitch, C.C., Silipino, L., Hadi, S., Weiss-Gayet, M., Barras, E., Schmid, C.D., Ait-Lounis, A., Barnes, A., Song, Y., Eisenman, D.J., Eliyahu, E., Frolenkov, G.I., Strome, S.E., Durand, B., Zaghloul, N.A., Jones, S.M., Reith, W., Hertzano, R. (2015) RFX transcription factors are essential for hearing in mice. Nature communications. 6:8549
- Wang, J., Wu, Y., Zhao, F., Wu, Y., Dong, W., Zhao, J., Zhu, Z., Liu, D. (2015) Fgf-signaling-dependent sox9a and atoh1a regulate otic neural development in zebrafish. The Journal of neuroscience : the official journal of the Society for Neuroscience. 35:234-44
- Tena, J.J., Alonso, M.E., de la Calle-Mustienes, E., Splinter, E., de Laat, W., Manzanares, M., and Gómez-Skarmeta, J.L. (2011) An evolutionarily conserved three-dimensional structure in the vertebrate Irx clusters facilitates enhancer sharing and coregulation. Nature communications. 2:310
- Choy, S.W., Cheng, C.W., Lee, S.T., Li, V.W., Hui, M.N., Hui, C.C., Liu, D., and Cheng, S.H. (2010) A cascade of irx1a and irx2a controls shh expression during retinogenesis. Developmental Dynamics : an official publication of the American Association of Anatomists. 239(12):3204-3214
- McDonald, L.A., Gerrelli, D., Fok, Y., Hurst, L.D., and Tickle, C. (2010) Comparison of Iroquois gene expression in limbs/fins of vertebrate embryos. Journal of anatomy. 216(6):683-691
- Foucher, I., Mione, M., Simeone, A., Acampora, D., Bally-Cuif, L., and Houart, C. (2006) Differentiation of cerebellar cell identities in absence of Fgf signalling in zebrafish Otx morphants. Development (Cambridge, England). 133(10):1891-1900
- Lecaudey, V., Anselme, I., Dildrop, R., Ruther, U., and Schneider-Maunoury, S. (2005) Expression of the zebrafish Iroquois genes during early nervous system formation and patterning. The Journal of comparative neurology. 492(3):289-302
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