PUBLICATION
Zebrafish parental progeny investment in response to cycling thermal stress and hypoxia: deposition of heat shock proteins but not cortisol
- Authors
- Lim, M.Y., Bernier, N.J.
- ID
- ZDB-PUB-221104-6
- Date
- 2022
- Source
- The Journal of experimental biology 225(21): (Journal)
- Registered Authors
- Keywords
- Cellular stress response, Diel cycling, Environmental stressors, Fish, Glucocorticoids, Non-genetic inheritance
- MeSH Terms
-
- Animals
- Female
- HSP70 Heat-Shock Proteins
- HSP90 Heat-Shock Proteins
- Heat-Shock Proteins*
- Hydrocortisone/metabolism
- Hypoxia
- Zebrafish*/metabolism
- PubMed
- 36326068 Full text @ J. Exp. Biol.
Citation
Lim, M.Y., Bernier, N.J. (2022) Zebrafish parental progeny investment in response to cycling thermal stress and hypoxia: deposition of heat shock proteins but not cortisol. The Journal of experimental biology. 225(21):.
Abstract
The maternal match hypothesis predicts that maternal exposure to a stressor may help prepare offspring to cope with the same disturbance in later life. Although there is support for this hypothesis, the signals involved in non-genetic inheritance are unclear. In this study we tested how adult zebrafish exposure to diel cycles of thermal stress (27-36°C), hypoxia (20-85% dissolved oxygen), or the combined treatment affect maternal and embryonic levels of cortisol and heat shock proteins (HSPs). While parental exposure to the thermal, hypoxic, or combined treatment for 2 weeks did not affect whole-body cortisol levels, the combined exposure increased ovarian cortisol levels by 4-fold and reduced embryonic cortisol content by 60%. The combined treatment also elicited 3- and 19-fold increases in embryo transcripts involved in cortisol breakdown (11bhsd2) and export (abcb4), respectively. The thermal stress and combined exposure also elicited marked increases in ovary and embryo hsp70a (20- to 45-fold) and HSP70 (3- to 7-fold), and smaller increases in ovary and embryo hsp90aa and hsp47 (2- to 4-fold) and in embryo HSP90 and HSP47 (2- to 6-fold). In contrast, except for increases in ovary hsp90aa (2-fold) and embryo HSP90 (3-fold), the hypoxia treatment had little effect on HSP expression and transfer. Overall, while the embryonic deposition of HSPs largely paralleled the ovarian cellular stress response, the inverse relationship between ovary and embryo cortisol levels suggests barriers against cortisol deposition in response to environmental stressors. We conclude that the endocrine and cellular stress responses make stressor-specific and distinct contributions to non-genetic inheritance.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping