PUBLICATION
Inhibition in growth and cardiotoxicity of tris (2-butoxyethyl) phosphate through down-regulating Wnt signaling pathway in early developmental stage of zebrafish (Danio rerio)
- Authors
- Xiong, H., Huang, Y., Mao, Y., Liu, C., Wang, J.
- ID
- ZDB-PUB-201020-57
- Date
- 2020
- Source
- Ecotoxicology and environmental safety 208: 111431 (Journal)
- Registered Authors
- Keywords
- Cardiotoxicity, Developmental toxicity, Oxidative stress, Tris (2-butoxyethyl) phosphate, Wnt signaling pathway, Zebrafish larvae
- MeSH Terms
-
- Phosphates/metabolism
- Zebrafish/metabolism
- Zebrafish/physiology*
- Animals
- Down-Regulation
- Plasticizers/toxicity*
- In Situ Nick-End Labeling
- Organophosphates/metabolism
- Water Pollutants, Chemical/metabolism
- Water Pollutants, Chemical/toxicity*
- Cardiotoxicity
- Flame Retardants/metabolism
- Larva/drug effects
- Embryo, Nonmammalian/drug effects
- Wnt Signaling Pathway/physiology
- Organophosphorus Compounds/metabolism
- Organophosphorus Compounds/toxicity*
- PubMed
- 33069947 Full text @ Ecotoxicol. Environ. Saf.
Citation
Xiong, H., Huang, Y., Mao, Y., Liu, C., Wang, J. (2020) Inhibition in growth and cardiotoxicity of tris (2-butoxyethyl) phosphate through down-regulating Wnt signaling pathway in early developmental stage of zebrafish (Danio rerio). Ecotoxicology and environmental safety. 208:111431.
Abstract
As a common organophosphorus flame retardant, tris (2-butoxyethyl) phosphate (TBOEP) is detected in water environment and aquatic animals extensively. Despite previous researches have reported the developmental toxicity of TBOEP in zebrafish (Danio rerio) larvae, few research focused on its underlying mechanisms. In this study, zebrafish embryos were exposed to 0, 20, 200, 1000 and 2000 µg/L TBOEP from 2 until 120 h post-fertilization (hpf) to determine potential mechanisms of developmental toxicity of this compound. Early developmental stage parameters such as body length, survival rate, hatching rate and heart rate were decreased, while malformation rate was ascended. Quantitative reverse transcription polymerase chain reaction (qRT-PCR) assay was carried out at 12, 24, 72 and 120 hpf to demonstrate alterations in expression of genes of Wnt signaling pathway. The results indicated that axin1 was significantly up-regulated, while β-catenin, pkc and wnt11 were down-regulated. Correlation analysis indicated that expression of these genes was significantly correlated with body length. Furthermore, apoptosis was detected in heart region by acridine orange (AO) staining and terminal deoxynucleotide transferase-mediated deoxy-UTP nick end labeling (TUNEL) assay. In addition, at 120 hpf, occurrence of oxidative stress was observed in zebrafish larvae. Moreover, 6-Bromoindirubin-3'-oxime (BIO)， an activator of Wnt pathway, was found to alleviate the inhibiting effects of TBOEP on zebrafish growth. The overall outcomes offered novel viewpoints in toxic effects of TBOEP, and down-regulating Wnt signaling pathway were able to reveal some potential mechanisms of developmental toxicity of TBOEP in zebrafish larvae.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping