PUBLICATION

Negative Feedback Regulation of Wnt4 Signaling by EAF1 and EAF2/U19

Authors
Wan, X., Ji, W., Mei, X., Zhou, J., Liu, J.X., Fang, C., and Xiao, W.
ID
ZDB-PUB-100223-40
Date
2010
Source
PLoS One   5(2): e9118 (Journal)
Registered Authors
Ji, Wei, Liu, Jing-xia, Wan, Xiaoyang, Xiao, Wuhan
Keywords
Zebrafish, Embryos, HEK 293 cells, Luciferase, Xenopus, Immunoprecipitation, Embryogenesis, Messenger RNA
MeSH Terms
  • Animals
  • Cell Line
  • Chromatin Immunoprecipitation
  • Embryo, Nonmammalian/embryology
  • Embryo, Nonmammalian/metabolism
  • Feedback, Physiological/physiology*
  • Female
  • Gene Expression Regulation, Developmental
  • Gene Knockdown Techniques
  • Humans
  • In Situ Hybridization
  • Male
  • Promoter Regions, Genetic/genetics
  • Protein Binding
  • Reverse Transcriptase Polymerase Chain Reaction
  • Signal Transduction*
  • Transcription Factors/genetics
  • Transcription Factors/metabolism
  • Transfection
  • Wnt Proteins/genetics
  • Wnt Proteins/metabolism*
  • Wnt4 Protein
  • Xenopus Proteins
  • Zebrafish/embryology
  • Zebrafish/genetics
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism*
PubMed
20161747 Full text @ PLoS One
Abstract
Previous studies indicated that EAF (ELL-associated factor) family members, EAF1 and EAF2/U19, play a role in cancer and embryogenesis. For example, EAF2/U19 may serve as a tumor suppressor in prostate cancer. At the same time, EAF2/U19 is a downstream factor in the non-canonical Wnt 4 signaling pathway required for eye development in Xenopus laevis, and along with EAF1, contributes to convergence and extension movements in zebrafish embryos through Wnt maintenance. Here, we used zebrafish embryos and mammalian cells to show that both EAF1 and EAF2/U19 were up-regulated by Wnt4 (Wnt4a). Furthermore, we found that EAF1 and EAF2/U19 suppressed Wnt4 expression by directly binding to the Wnt4 promoter as seen in chromatin immunoprecipitation assays. These findings indicate that an auto-regulatory negative feedback loop occurs between Wnt4 and the EAF family, which is conserved between zebrafish and mammalian. The rescue experiments in zebrafish embryos showed that early embryonic development required the maintenance of the appropriate levels of Wnt4a through the feedback loop. Others have demonstrated that the tumor suppressors p63, p73 and WT1 positively regulate Wnt4 expression while p21 has the opposite effect, suggesting that maintenance of appropriate Wnt4 expression may also be critical for adult tissue homeostasis and prevention against tumor initiation. Thus, the auto-regulatory negative feedback loop that controls expression of Wnt4 and EAF proteins may play an important role in both embryonic development and tumor suppression. Our findings provide the first convincing line of evidence that EAF and Wnt4 form an auto-regulatory negative feedback loop in vivo.
Genes / Markers
Figures
Show all Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping