PUBLICATION
Interaction with surrounding normal epithelial cells influences signalling pathways and behaviour of Src-transformed cells
- Authors
- Kajita, M., Hogan, C., Harris, A.R., Dupre-Crochet, S., Itasaki, N., Kawakami, K., Charras, G., Tada, M., and Fujita, Y.
- ID
- ZDB-PUB-100105-11
- Date
- 2010
- Source
- Journal of Cell Science 123(Pt 2): 171-180 (Journal)
- Registered Authors
- Kawakami, Koichi, Tada, Masazumi
- Keywords
- Apical extrusion, Focal adhesion kinase, Mitogen-activated protein kinase, Myosin-II, Src, Zebrafish
- MeSH Terms
-
- Animals
- Cadherins/metabolism
- Cell Adhesion
- Cell Communication*
- Cell Polarity
- Cell Transformation, Neoplastic/metabolism*
- Cell Transformation, Neoplastic/pathology*
- Dogs
- Epithelial Cells/cytology*
- Epithelial Cells/metabolism*
- Focal Adhesion Protein-Tyrosine Kinases/metabolism
- Humans
- Mitogen-Activated Protein Kinases/metabolism
- Models, Biological
- Myosin Type II/metabolism
- Oncogene Protein pp60(v-src)/metabolism*
- Protein Transport
- Signal Transduction*
- Zebrafish/metabolism
- beta Catenin/metabolism
- PubMed
- 20026643 Full text @ J. Cell Sci.
Citation
Kajita, M., Hogan, C., Harris, A.R., Dupre-Crochet, S., Itasaki, N., Kawakami, K., Charras, G., Tada, M., and Fujita, Y. (2010) Interaction with surrounding normal epithelial cells influences signalling pathways and behaviour of Src-transformed cells. Journal of Cell Science. 123(Pt 2):171-180.
Abstract
At the initial stage of carcinogenesis, transformation occurs in a single cell within an epithelial sheet. However, it remains unknown what happens at the boundary between normal and transformed cells. Using Madin-Darby canine kidney (MDCK cells transformed with temperature-sensitive v-Src, we have examined the interface between normal and Src-transformed epithelial cells. We show that Src-transformed cells are apically extruded when surrounded by normal cells, but not when Src cells alone are cultured, suggesting that apical extrusion occurs in a cell-context-dependent manner. We also observe apical extrusion of Src-transformed cells in the enveloping layer of zebrafish gastrula embryos. When Src-transformed MDCK cells are surrounded by normal MDCK cells, myosin-II and focal adhesion kinase (FAK are activated in Src cells, which further activate downstream mitogen-activated protein kinase (MAPK. Importantly, activation of these signalling pathways depends on the presence of surrounding normal cells and plays a crucial role in apical extrusion of Src cells. Collectively, these results indicate that interaction with surrounding normal epithelial cells influences the signalling pathways and behaviour of Src-transformed cells.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping