PUBLICATION
A genetic linkage map for zebrafish: comparative analysis and localization of genes and expressed sequences
- Authors
- Gates, M.A., Kim, L., Egan, E.S., Cardozo, T., Sirotkin, H.I., Dougan, S.T., Lashkari, D., Abagyan, R., Schier, A.F., and Talbot, W.S.
- ID
- ZDB-PUB-990507-7
- Date
- 1999
- Source
- Genome research 9(4): 334-347 (Journal)
- Registered Authors
- Dougan, Scott T., Egan, Elizabeth S., Gates, Michael A., Schier, Alexander, Sirotkin, Howard, Talbot, William S.
- Keywords
- none
- MeSH Terms
-
- Animals
- Chromosomes, Human
- Female
- Genetic Linkage*
- Humans
- Male
- Molecular Sequence Data
- Mutation
- Phylogeny
- Physical Chromosome Mapping/methods*
- Zebrafish/genetics*
- PubMed
- 10207156 Full text @ Genome Res.
Citation
Gates, M.A., Kim, L., Egan, E.S., Cardozo, T., Sirotkin, H.I., Dougan, S.T., Lashkari, D., Abagyan, R., Schier, A.F., and Talbot, W.S. (1999) A genetic linkage map for zebrafish: comparative analysis and localization of genes and expressed sequences. Genome research. 9(4):334-347.
Abstract
Genetic screens in zebrafish (Danio rerio) have isolated mutations in hundreds of genes with essential functions. To facilitate the identification of candidate genes for these mutations, we have genetically mapped 104 genes and expressed sequence tags by scoring single-strand conformational polymorphisms in a panel of haploid siblings. To integrate this map with existing genetic maps, we also scored 275 previously mapped genes, microsatellites, and sequence-tagged sites in the same haploid panel. Systematic phylogenetic analysis defined likely mammalian orthologs of mapped zebrafish genes, and comparison of map positions in zebrafish and mammals identified significant conservation of synteny. This comparative analysis also identified pairs of zebrafish genes that appear to be orthologous to single mammalian genes, suggesting that these genes arose in a genome duplication that occurred in the teleost lineage after the divergence of fish and mammal ancestors. This comparative map analysis will be useful in predicting the locations of zebrafish genes from mammalian gene maps and in understanding the evolution of the vertebrate genome.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping