(A) Daily rotifer concentration delivered per growth treatment, excluding weekends (day 8 & 9). Open circles = LF, closed squares = HF. (B) Daily Artemia nauplii delivered per growth treatment, excluding weekends. Open circles = LF, closed squares = HF.

Mean total body length for two F₀ generation wild-type strains, (A) AB and (B) Tü. Growth curves are represented by mean ± standard deviation. Open circles = LF, closed squares = HF.

Reproductive performance for the F₀ (A, B) AB wild-type strain and (C, D) Tü wild-type line. (A) Fecundity is defined as the total number of live embryos spawned and (B) viability is defined as the percentage of live embryos at 24-hours post fertilization. Data shown as the mean ± standard deviation. Significant differences at each week are noted with * indicating p ≤ 0.05, ** indicating p ≤ 0.01, and *** indicating p ≤ 0.001 by successive two-tailed T-Test, n = 3 represented by 30 fish from each growth treatment.

Growth curves for two F₁ generation wildtype lines (offspring of F₀), (A) AB and (B) Tü from 5 to 90 DPF. Slow growth curves are represented by mean ± standard deviation. Open circles = LF from F₀ LF parents, closed squares = LF from F₀ HF parents.

Reproductive performance for the F₁ (A, B) AB wild-type strain and (C, D) Tü wild-type strain. (A) Fecundity defined as the total number of live embryos spawned and (B) viability defined as the percentage of live embryos at 24-hours post fertilization. Data shown as the mean ± standard deviation. No significant differences were measured in either fecundity or viability, n = 3 represented by 30 fish from each growth treatment.

Embryo viability measured from the F₀ and F₁ generations were used to calculate the fold change of the LF/HF in both wild-type strains (A) AB, (B) Tü. Values that deviate from 1.0 demonstrate a greater difference in reproductive performance.