- Title
-
Assessment of Biofilm Formation by Candida albicans Strains Isolated from Hemocultures and Their Role in Pathogenesis in the Zebrafish Model
- Authors
- Pokhrel, S., Boonmee, N., Tulyaprawat, O., Pharkjaksu, S., Thaipisutikul, I., Chairatana, P., Ngamskulrungroj, P., Mitrpant, C.
- Source
- Full text @ J Fungi (Basel)
Survival of zebrafish larvae injected with either C. albicans strains 104 (non-biofilm former), 57 (weak biofilm former), or 140 (strong biofilm former). The control group was injected with PBS without an organism. Each experiment was performed in duplicate. |
Fungal tissue burden in zebrafish larvae that were infected with yeast cells of C. albicans strains 104, 57, or 140. Each experiment was performed in triplicate. CFU, colony forming unit. |
The expression levels of eap1, als3, hwp1, bcr1, and mkc1 in C. albicans strains 104, 57, and 140 infected zebrafish larvae, by time point. Each experimental group of 30 zebrafish was injected with 0.7 OD yeast cells of one of the C. albicans strains. The normalized ratios of expression were calculated by comparison with the level of expression of the act-1 gene in each group of planktonic cells. Each experiment was performed in duplicate. h, hour; **, p < 0.01; ***, p < 0.001; and ****, p < 0.0001. |