Fig. 1. PDMS microreactor used to synthesize QDs: a) Schematic along with dimensions b) Optical image. c) Schematic of the setup. d) Chemical reactions involved in the synthesis of PDMS coated CdTe quantum dots.

Fig. 2. AFM 3D topographic images and size distribution graph different sized QDs of a) 0.5 nm; b) 3.32 nm; c) 3.34 nm; and d) 3.92 nm; e) Photoluminescence spectra of all the QDs; f) TEM of green-colored CdTe nanoparticles are seen in a size range of 3–5 nm (atomic planes are visible in the inset image).

Fig. 3. a) FT-IR spectra of bare CdTe and PDMS coated CdTe QDs. b) TEM showing uniformly dispersed P-QDs with the size of ~3–5 nm. The inset of the image shows an enlarged image revealing atomic planes. c) Photoluminescence spectra recorded at an excitation wavelength of 320 nm. The background picture shows different colored P-QDs.

Fig. 4. a) Cytotoxicity studies with NIH 3T3 cells indicate biocompatibility of P-QDs at a dose of 6.25 μg/mL b) Time-dependent ion leaching studies show low Cd²⁺ release from P-QDs as compared to bare counterparts.

Fig. 5. Confocal microscopic images of conventional and microreactor synthesized QDs internalized in HepG2 cells. DAPI is used for staining the nucleus. The excitation wavelength used for visualizing DAPI and QDs was 405 nm. Scale bar: 50 μm; Magnification: 63×.

Fig. 6. Confocal microscopic images of NIH 3T3 cells. Hoechst 33342 (360/460) and Actin Green (495/518) were used as a control to stain the nucleus and cytoskeleton, respectively, and captured sequentially. Blue and green QDs were conjugated with SMAR-1 nucleus-specific protein and smooth muscle actin antibodies to target and cytoskeleton, respectively. The bottom panel was captured simultaneously at an excitation wavelength of 405 nm. Scale bar: 10 μm; Magnification: 63×.

Fig. 7. Confocal images of sagittal cardiac sections showing cardiomyocytes and proliferating cells in wild-type zebrafish cardiac tissue. The dye control panel shows Alexa Fluor 488-phalloidin and Alexa Fluor 647-phalloidin conjugated with primary antibodies of MF20 and PCNA, respectively. The overlay image shows Alexa Fluor 555 Phalloidin (Green) and Alexa Fluor 647-phalloidin conjugated with PCNA primary antibody. A comparison of fluorescence intensities of QDs and dyes analyzed using ImageJ software is shown as a bar chart. PQDs control panel represents confocal images stained with unconjugated green and red P-QDs as a negative control. Green P-QDs conjugated MF20, and red P-QDs conjugated PCNA recognize cardiomyocytes and nuclei undergoing DNA synthesis. Arrowheads point to the proliferating cells in the injury region (for dye control and QDs). The yellow dotted line (for dye control and QDs) demarcates the healthy cardiac tissue from the cryo-injured region. The images with the dye were captured after 4 dpci, whereas those with QDs were imaged after 7 dpci. Scale bar: 50 μm; Magnification: 63×.