Depletion of Kpna4 did not affect the overall development of zebrafish. (A) Schematic illustration of the position of the kpna4 gene edited by sgRNA. (B) The kpna4 knockout zebrafish were genotyped with PCR, followed by BbvI digestion. (C) Sanger sequencing results showed 1 bp was substituted and 4 bp deleted in kpna4 knockout zebrafish. (D) The larvae of 72 hpf WT and kpna4 knockout zebrafish.

Lens defects in kpna4 knockout zebrafish. (A) Depletion of Kpna4 caused cataract-like phenotype in 72 hpf zebrafish. (B) Electron micrographs of lens epithelium (LE) and lens fiber (LF) in 72 hpf WT and kpna4 knockout zebrafish.

Gene expression and PPI of KPNA4 in human and mice database. (A) Lens-enrichment of KPNA4 at different developmental stages in human genome hg38 assembly using the UCSC Genome Browser. (B) KPNA4 gene expression from GeneAtlas MOE430, gcrma dataset with eye tissues in mice. (C) A PPI network of KPNA4 gene with 106 nodes and 338 edges was obtained though BioGRID database. (D) KPNA4 and p53 had an interaction score >0.4 according to the STRING database.

Hydrogen peroxide induced apoptosis in HLE cells. (A) HLE cells morphology changed after treated by different does of H₂O₂. (B) The apoptotic HLE cells were detected by flow cytometry after treated by different does of H₂O₂.

The expression pattern of p53 in H₂O₂-induced apoptotic cells and kpna4 knockout zebrafish. (A) HLE cells stained for KPNA4 (green) and p53 (red) in the normal control (NC) group or 200 μmol/L H₂O₂ treatment. (B-C) Western blot with KPNA4 and p53 in the NC and 200 μmol/L H₂O₂ treatment group in HLE cells or 293 T cells. (D-E) The relative intensity of KPNA4/actin and p53/actin of three independent experiments in HLE cells or 293 T cells. (F) Immunofluorescence for p53 in the lens of 72 hpf WT and kpna4 knockout zebrafish. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)