Abundant DNA 6mA methylation during early embryogenesis of zebrafish and pig

Liu, J., Zhu, Y., Luo, G.Z., Wang, X., Yue, Y., Wang, X., Zong, X., Chen, K., Yin, H., Fu, Y., Han, D., Wang, Y., Chen, D., He, C.
Full text @ Nat. Commun.

6mA modification in gDNAs of gametes and selected early embryos in both zebrafish and pig

(a,c) Quantification of 6mA modification in isolated gDNA from sperm, oocyte and various embryo stages of zebrafish (a) and pig (c) by UHPLC–QQQ–MS/MS. The mole ratios of 6mA/A are shown, error bars indicate mean±s.d. (n=3). (b,d) Immunofluorescence images of selected embryo stages of zebrafish (b) and pig (d) at single-cell level stained by anti-6mA antibody (green, rabbit polyclonal from SYSY) and anti-Histone 3 (H3) antibody (red, mouse monoclonal from Biodragon Immunotechnologies). Early embryo stages show strong fluorescence indicative of the high abundance of 6mA in gDNA, whereas the signal is diminished with the progression of the embryo development. Full embryo images are presented in Supplementary Fig. 3.

Anatomical Terms:
Stage Range: 1-cell to Adult

(a, c) Global view of immunofluorescence images of selected embryo stages of zebrafish (a) and pig (c) stained by anti-6mA antibody. The dash line-circled area delineates the zebrafish sperm. (b) Immunostaining of zebrafish genomic DNA 6mA at 64C stage with and without DNase treatment. Histone 3 (H3) staining was used as counterstain. The scale bars are as indicated.

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