Two-dimensional gel electrophoresis of total zebrafish lens protein shows age-specific expression patterns. Separation was performed on 11 cm pH gradient 3–10 nonlinear immobilized pH gradient strips. Ovals indicate the location of different crystallin groups, and labels note their first appearance. Alpha-crystallins are shown in blue and β/γ-crystallins in green. Black arrows indicate phosphorylated αA-crystallin [31]. Spot identifications relied on previously published proteomics maps [31] and were confirmed with matrix-assisted laser desorption/ionization time of flight (MALDI-TOF) mass spectrometry. Gels were stained with SYPRO Ruby Red.

Separation of total zebrafish lens protein using 7–10 pH strips identifies ontogenetic changes in β/γ-crystallin expression. The identities of several crystallins found in each gel are noted. Numbered ovals indicate protein spots found only in the 6- and 10-week lenses.